Abstract
To better understand the role of nicotinic acid and nicotinamide in the regulation of the oxidative stress response, we measured the levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glucose-6-phosphate dehydrogenase (GGPD) mRNA in Jurkat cells treated with these NAD+ precursors. We used a modified nonradioactive Northern blot method and detected the mRNA using 18-mer digoxigenin (DIG)-labeled oligonucleotides as probes. We observed increased levels of the mRNAs for the two enzymes in treated cells. Our findings suggest that the NAD+ precursors may protect against oxidative stress and DNA damage by up-regulating the stress response genes GAPDH and G6PD.
Original language | English (US) |
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Pages (from-to) | 133-136 |
Number of pages | 4 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 255 |
Issue number | 1 |
DOIs | |
State | Published - Feb 5 1999 |
Keywords
- Glucose-6-phosphate dehydrogenase
- Glyceraldehyde-3-phosphate dehydrogenase
- Nicotinamide
- Nicotinic acid
- Oxidative stress
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology