The mRNA encoding elongation factor 1-α (EF-1α) is a major transcript at the midblastula transition in Xenopus

Paul A. Krieg; Susan M. Varnum; W. Michael Wormington; Doug A. Melton

doi:10.1016/0012-1606(89)90300-X

The mRNA encoding elongation factor 1-α (EF-1α) is a major transcript at the midblastula transition in Xenopus

Paul A. Krieg, Susan M. Varnum, W. Michael Wormington, Doug A. Melton

Cellular and Molecular Medicine

Research output: Contribution to journal › Article › peer-review

402 Scopus citations

Abstract

A Xenopus laevis gastrula cDNA library has been screened in order to identify sequences that are expressed early in development. We find that the mRNA encoding translation elongation factor 1-α (EF-1α) is synthesized in very large amounts in the early embryo. Transcription of EF-1α mRNA commences at the midblastula transition (MBT), and new EF-1α protein is synthesized very soon after this, as determined by the association of EF-1α mRNA with polysomes. The nucleotide sequence of a full-length EF-1α cDNA clone and the deduced amino acid sequence of Xenopus EF-1α protein are presented.

Original language	English (US)
Pages (from-to)	93-100
Number of pages	8
Journal	Developmental biology
Volume	133
Issue number	1
DOIs	https://doi.org/10.1016/0012-1606(89)90300-X
State	Published - May 1989

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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10.1016/0012-1606(89)90300-X

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title = "The mRNA encoding elongation factor 1-α (EF-1α) is a major transcript at the midblastula transition in Xenopus",

abstract = "A Xenopus laevis gastrula cDNA library has been screened in order to identify sequences that are expressed early in development. We find that the mRNA encoding translation elongation factor 1-α (EF-1α) is synthesized in very large amounts in the early embryo. Transcription of EF-1α mRNA commences at the midblastula transition (MBT), and new EF-1α protein is synthesized very soon after this, as determined by the association of EF-1α mRNA with polysomes. The nucleotide sequence of a full-length EF-1α cDNA clone and the deduced amino acid sequence of Xenopus EF-1α protein are presented.",

author = "Krieg, {Paul A.} and Varnum, {Susan M.} and Wormington, {W. Michael} and Melton, {Doug A.}",

note = "Funding Information: This work was supported by grants from the NIH to W.M.W. and to D.A.M. and from the Chicago Community Trust/Searle Scholars program (D.A.M.). P.A.K. is grateful for support from the Fogarty International Research Fellowship Program and from the CSIRO.",

year = "1989",

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doi = "10.1016/0012-1606(89)90300-X",

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AU - Varnum, Susan M.

AU - Wormington, W. Michael

AU - Melton, Doug A.

N1 - Funding Information: This work was supported by grants from the NIH to W.M.W. and to D.A.M. and from the Chicago Community Trust/Searle Scholars program (D.A.M.). P.A.K. is grateful for support from the Fogarty International Research Fellowship Program and from the CSIRO.

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N2 - A Xenopus laevis gastrula cDNA library has been screened in order to identify sequences that are expressed early in development. We find that the mRNA encoding translation elongation factor 1-α (EF-1α) is synthesized in very large amounts in the early embryo. Transcription of EF-1α mRNA commences at the midblastula transition (MBT), and new EF-1α protein is synthesized very soon after this, as determined by the association of EF-1α mRNA with polysomes. The nucleotide sequence of a full-length EF-1α cDNA clone and the deduced amino acid sequence of Xenopus EF-1α protein are presented.

AB - A Xenopus laevis gastrula cDNA library has been screened in order to identify sequences that are expressed early in development. We find that the mRNA encoding translation elongation factor 1-α (EF-1α) is synthesized in very large amounts in the early embryo. Transcription of EF-1α mRNA commences at the midblastula transition (MBT), and new EF-1α protein is synthesized very soon after this, as determined by the association of EF-1α mRNA with polysomes. The nucleotide sequence of a full-length EF-1α cDNA clone and the deduced amino acid sequence of Xenopus EF-1α protein are presented.

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The mRNA encoding elongation factor 1-α (EF-1α) is a major transcript at the midblastula transition in Xenopus

Abstract

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