@article{822445bb88b240a89b234ef8c2d3dd21,
title = "The minimum element of a synthetic peptide required to block prostate tumor cell migration",
abstract = "Human prostate tumor cell invasion and metastasis are dependent in part on cell adhesion to extracellular matrix proteins and cell migration. We previously identified a synthetic D-amino acid tumor cell adhesion peptide called HYD1 (kikmviswkg) that supported adhesion of tumor cells derived from breast, prostate, ovary and pancreas tissue. Alanine substitution analysis and a peptide deletion strategy were used to determine the minimal element of HYD1 necessary for bioactivity in a prostate cancer cell line called PC3N. Bioactivity was measured by assays of cell adhesion, migration and ERK signaling. The most potent element of HYD1 necessary to support cell adhesion was kmvixw, the block to migration required xkmviswxx and activation of ERK signaling required ikmviswxx. The shortest sequence active in all three assays was iswkg. The HYD1 peptide contains overlapping elements required for adhesion, blocking migration and the activation of ERK signaling. These linear peptide sequences provide the starting point for development of novel compounds to target cancer cell adhesion and migration.",
keywords = "Cell adhesion, ERK signaling, Migration, Prostate cancer, Synthetic peptide",
author = "Sroka, {Thomas C.} and Jan Marik and Pennington, {Michael E.} and Lam, {Kit S.} and Cress, {Anne E.}",
note = "Funding Information: Multiple studies have isolated biologically active peptides from defined regions within laminin chains and documented profound effects on biological events including cell migration and metastasis.11-18The discovery of RGD, the tripeptide sequence found in many adhesive proteins such as fibronectin and vitronectin,19,20 has led to several studies showing that this cell adhesion peptide has anti-invasive and anti-metastatic effects both in vitro and in vivo.21,22 Peptides derived from the laminin β1 chain, YIGSR, and α5 by: NIH, T32 CA 09213, CA75152, CAThe work was supported by grants provided chain, RLVSYNGIIFFLK, have also been effective at blocking experimental metas-23074, CA56666 and Department of Defense tasis.11,18,23 An alternative to identifying biologically active regions from extracellular Program Grant DAMD17-03-1-0110 to J.M. matrix proteins is to develop synthetic ligands using combinatorial chemistry techniques. We appreciate the helpful discussions of the The one-bead-one-compound combinatorial library method (OBOC)24-26 and the results with Drs. Bowden, Nagle and Vagner phage-display peptide library approach27-30 have been successfully used to identify peptide and the technical assistance of D{\textcopyright} r. Man Ling ligands for cell surface molecules. These techniques have the potential to produce novel Chen. peptides with antagonistic effects on the targeted cell surface receptor.",
year = "2006",
month = nov,
doi = "10.4161/cbt.5.11.3461",
language = "English (US)",
volume = "5",
pages = "1556--1562",
journal = "Cancer Biology and Therapy",
issn = "1538-4047",
publisher = "Taylor and Francis Ltd.",
number = "11",
}