Abstract
Context: The lipid peroxidation end-product and oxidant 4-hydroxynonenal (4-HNE) impairs cell function. However, the impact of 4-HNE on the glucose transport system in mammalian slow-twitch skeletal muscle is not known. Objective: We assessed the effects of 4-HNE on insulin signalling and glucose transport activity in slow-twitch muscle by incubating soleus strips from lean Zucker rats with 4-HNE (50 μM) in the absence or presence of insulin (5 mU/ml) for up to 6 hr. Results: Insulin-stimulated glucose transport activity was significantly (p < 0.05) decreased by 4-HNE at 2 hr. AS160 Thr 642 phosphorylation was decreased at 2 hr, whereas Akt Ser 473 phosphorylation and IRS-1 protein expression were not substantially changed until 4 hr. IRS-2 protein expression was slightly decreased only at 6 hr. Conclusions: The lipid peroxidation end-product and oxidant 4-HNE induces insulin resistance of glucose transport activity in rat slow-twitch skeletal muscle, initially associated with impaired phosphorylation of AS160.
Original language | English (US) |
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Pages (from-to) | 22-28 |
Number of pages | 7 |
Journal | Archives of Physiology and Biochemistry |
Volume | 120 |
Issue number | 1 |
DOIs | |
State | Published - Feb 2014 |
Keywords
- AS160 phosphorylation
- Glucose transport
- Lean Zucker rat
- Oxidative stress
- Soleus muscle
ASJC Scopus subject areas
- Physiology
- Physiology (medical)