TY - JOUR
T1 - The K/BxN mouse model of inflammatory arthritis
T2 - theory and practice.
AU - Monach, Paul
AU - Hattori, Kimie
AU - Huang, Haochu
AU - Hyatt, Elzbieta
AU - Morse, Jody
AU - Nguyen, Linh
AU - Ortiz-Lopez, Adriana
AU - Wu, Hsin Jung
AU - Mathis, Diane
AU - Benoist, Christophe
PY - 2007
Y1 - 2007
N2 - Mice expressing the KRN T cell receptor transgene and the MHC class II molecule A(g7) (K/BxN mice) develop severe inflammatory arthritis, and serum from these mice causes similar arthritis in a wide range of mouse strains, owing to pathogenic autoantibodies to glucose-6-phosphate isomerase (GPI). This model has been useful for the investigation of the development of autoimmunity (K/BxN transgenic mice) and particularly of the mechanisms by which anti-GPI autoantibodies induce joint-specific imflammation (serum transfer model). In this chaper, after a summary of findings from this model system, we describe detailed methods for the maintenance of a K/BxN colony, crossing of the relevant TCR and MHC genes to other strain backgrounds, evaluation of KRN transgenic T cells, measurement of anti-GPI antibodies, induction of arthritis by serum transfer, and clinical and histological evaluation of arthritis.
AB - Mice expressing the KRN T cell receptor transgene and the MHC class II molecule A(g7) (K/BxN mice) develop severe inflammatory arthritis, and serum from these mice causes similar arthritis in a wide range of mouse strains, owing to pathogenic autoantibodies to glucose-6-phosphate isomerase (GPI). This model has been useful for the investigation of the development of autoimmunity (K/BxN transgenic mice) and particularly of the mechanisms by which anti-GPI autoantibodies induce joint-specific imflammation (serum transfer model). In this chaper, after a summary of findings from this model system, we describe detailed methods for the maintenance of a K/BxN colony, crossing of the relevant TCR and MHC genes to other strain backgrounds, evaluation of KRN transgenic T cells, measurement of anti-GPI antibodies, induction of arthritis by serum transfer, and clinical and histological evaluation of arthritis.
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U2 - 10.1007/978-1-59745-402-5_20
DO - 10.1007/978-1-59745-402-5_20
M3 - Article
C2 - 17983155
AN - SCOPUS:38449104460
SN - 1543-1894
VL - 136
SP - 269
EP - 282
JO - Methods in molecular medicine
JF - Methods in molecular medicine
ER -