The intracellular proton gradient enables anaerobic ammonia oxidizing (anammox) bacteria to tolerate NO2- inhibition

José M. Carvajal-Arroyo; Daniel Puyol; Guangbin Li; Reyes Sierra-Álvarez; Jim A. Field

doi:10.1016/j.jbiotec.2014.10.032

The intracellular proton gradient enables anaerobic ammonia oxidizing (anammox) bacteria to tolerate NO₂^- inhibition

José M. Carvajal-Arroyo, Daniel Puyol, Guangbin Li, Reyes Sierra-Álvarez, Jim A. Field

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Anammox bacteria are inhibited by nitrite, which is one of their substrates. By utilizing 2,4 dinitrophenol and carbonyl cyanide m-chlorophenyl hydrazone, two uncouplers of respiration, we demonstrate that nitrite tolerance of anammox cells is strongly dependent on their ability to maintain a proton gradient, which may be the driving force for active nitrite transport system.

Original language	English (US)
Pages (from-to)	265-267
Number of pages	3
Journal	Journal of Biotechnology
Volume	192
Issue number	Part A
DOIs	https://doi.org/10.1016/j.jbiotec.2014.10.032
State	Published - Dec 1 2014

Keywords

Active transport
Biofilm
Detoxification
Toxicity
Uncoupler

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

Access to Document

10.1016/j.jbiotec.2014.10.032

Cite this

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title = "The intracellular proton gradient enables anaerobic ammonia oxidizing (anammox) bacteria to tolerate NO2- inhibition",

abstract = "Anammox bacteria are inhibited by nitrite, which is one of their substrates. By utilizing 2,4 dinitrophenol and carbonyl cyanide m-chlorophenyl hydrazone, two uncouplers of respiration, we demonstrate that nitrite tolerance of anammox cells is strongly dependent on their ability to maintain a proton gradient, which may be the driving force for active nitrite transport system.",

keywords = "Active transport, Biofilm, Detoxification, Toxicity, Uncoupler",

author = "Carvajal-Arroyo, {Jos{\'e} M.} and Daniel Puyol and Guangbin Li and Reyes Sierra-{\'A}lvarez and Field, {Jim A.}",

note = "Funding Information: This work has been supported by the University of Arizona Water Sustainability Program , and by the National Science Foundation (under Contract CBET-1234211). Publisher Copyright: {\textcopyright} 2014 Elsevier B.V.",

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T1 - The intracellular proton gradient enables anaerobic ammonia oxidizing (anammox) bacteria to tolerate NO2- inhibition

AU - Carvajal-Arroyo, José M.

AU - Puyol, Daniel

AU - Li, Guangbin

AU - Sierra-Álvarez, Reyes

AU - Field, Jim A.

N1 - Funding Information: This work has been supported by the University of Arizona Water Sustainability Program , and by the National Science Foundation (under Contract CBET-1234211). Publisher Copyright: © 2014 Elsevier B.V.

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Anammox bacteria are inhibited by nitrite, which is one of their substrates. By utilizing 2,4 dinitrophenol and carbonyl cyanide m-chlorophenyl hydrazone, two uncouplers of respiration, we demonstrate that nitrite tolerance of anammox cells is strongly dependent on their ability to maintain a proton gradient, which may be the driving force for active nitrite transport system.

AB - Anammox bacteria are inhibited by nitrite, which is one of their substrates. By utilizing 2,4 dinitrophenol and carbonyl cyanide m-chlorophenyl hydrazone, two uncouplers of respiration, we demonstrate that nitrite tolerance of anammox cells is strongly dependent on their ability to maintain a proton gradient, which may be the driving force for active nitrite transport system.

KW - Active transport

KW - Biofilm

KW - Detoxification

KW - Toxicity

KW - Uncoupler

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