The influence of extracellular matrix derived from skeletal muscle tissue on the proliferation and differentiation of myogenic progenitor cells ex vivo

Matthew M. Stern, Regina L. Myers, Nevin Hammam, Kathryn A. Stern, Daniel Eberli, Stephen B. Kritchevsky, Shay Soker, Mark Van Dyke

Research output: Contribution to journalArticlepeer-review

123 Scopus citations

Abstract

Skeletal muscle relies upon regeneration to maintain homeostasis and repair injury. This process involves the recruitment of the tissue's resident stem cell, the muscle progenitor cell, and a subsequent proliferative response by newly generated myoblasts, which must then align and fuse to generate new muscle fibers. During regeneration, cells rely on environmental input for direction. Extracellular matrix (ECM) represents a crucial component of a cell's microenvironment that aids in guiding muscle regeneration. We hypothesized that ECM extracted from skeletal muscle would provide muscle progenitor cells and myoblasts with an ideal substrate for growth and differentiation ex vivo. To test this hypothesis, we developed a method to extract ECM from the large thigh muscles of adult rats and present it to cells as a surface coating. Myogenic cells cultured on ECM extract experienced enhanced proliferation and differentiation relative to standard growth surfaces. As the methodology can be applied to any size muscle, these results demonstrate that bioactive ECM can be readily obtained from skeletal muscle and used to develop biomaterials that enhance muscle regeneration. Furthermore, the model system demonstrated here can be applied to the study of interactions between the ECM of a particular tissue and a cell population of interest.

Original languageEnglish (US)
Pages (from-to)2393-2399
Number of pages7
JournalBiomaterials
Volume30
Issue number12
DOIs
StatePublished - Apr 2009
Externally publishedYes

Keywords

  • Cell culture
  • Cell proliferation
  • Extracellular matrix
  • Muscle
  • Progenitor cell

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Ceramics and Composites
  • Biomaterials
  • Mechanics of Materials

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