The influence of external potassium ions upon lens conductance characteristics investigated using a voltage clamp technique

N. A. Delamere, C. A. Paterson, D. L. Holmes

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

An intracellular voltage clamp technique for the lens is described. Using this technique, it was possible to describe directly the conductance-voltage relationship of the lens over a wide range of lens potentials. The influence of potassium-rich solutions on lens conductance and 42K efflux rate was examined under conditions where the voltage clamp prevented changes in the lens potential. Under these conditions, potassium-rich solutions caused a reduction in lens conductance and a fall in the 42K efflux rate. These observations suggest that, at the resting potential, potassium-rich solutions influence conductance by decreasing lens potassium permeability. However, when the lens is not voltage clamped, the reduction of lens conductance is masked because the depolarization of lens potential increases the voltage-dependent conductance component, leading to an overall increase in the conductance of the lens.

Original languageEnglish (US)
Pages (from-to)651-658
Number of pages8
JournalExperimental eye research
Volume31
Issue number6
DOIs
StatePublished - Dec 1980

Keywords

  • lens
  • membrane conductance
  • potassium
  • voltage clamp

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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