The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex

Zênis Novais Da Rocha; Mario Sérgio Pereira Marchesi; Josiane Cristina Molin; Claure N. Lunardi; Katrina M. Miranda; Lusiane Maria Bendhack; Peter C. Ford; Roberto Santana Da Silva

doi:10.1039/b803441a

The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO₂)L(bpy)₂]⁺ complex

Zênis Novais Da Rocha
, Mario Sérgio Pereira Marchesi
, Josiane Cristina Molin
, Claure N. Lunardi
, Katrina M. Miranda
, Lusiane Maria Bendhack
, Peter C. Ford
, Roberto Santana Da Silva

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

The synthesis of [Ru(NO₂)L(bpy)₂]⁺ (bpy = 2,2′-bipyridine and L = pyridine (py) and pyrazine (pz)) can be accomplished by addition of [Ru(NO)L(bpy)₂](PF₆)₃ to aqueous solutions of physiological pH. The electrochemical processes of [Ru(NO₂)L(bpy)₂]⁺ in aqueous solution were studied by cyclic voltammetry and differential pulse voltammetry. The anodic scan shows a peak around 1.00 V vs. Ag/AgCl attributed to the oxidation process centered on the metal ion. However, in the cathodic scan a second peak around −0.60 V vs. Ag/AgCl was observed and attributed to the reduction process centered on the nitrite ligand. The controlled reduction potential electrolysis at −0.80 V vs. Ag/AgCl shows NO release characteristics as judged by NO measurement with a NO-sensor. This assumption was confirmed by ESI/MS⁺ and spectroelectrochemical experiment where cis-[Ru(bpy)₂L(H₂O)]²⁺ was obtained as a product of the reduction of cis-[Ru^II(NO₂)L(bpy)₂]⁺. The vasorelaxation observed in denuded aortic rings pre-contracted with 0.1 μmol L⁻¹ phenylephrine responded with relaxation in the presence of cis-[Ru^II(NO₂)L(bpy)₂]⁺. The potential of rat aorta cells to metabolize cis-[Ru^II(NO₂)L(bpy)₂]⁺ was also followed by confocal analysis. The obtained results suggest that NO release happens by reduction of cis-[Ru^II(NO₂)L(bpy)₂]⁺ inside the cell. The maximum vasorelaxation was achieved with 1 × 10⁻⁵ mol L⁻¹ of cis-[Ru^II(NO₂)L(bpy)₂]⁺ complex.

Original language	English (US)
Pages (from-to)	4282-4287
Number of pages	6
Journal	Journal of the Chemical Society. Dalton Transactions
Issue number	32
DOIs	https://doi.org/10.1039/b803441a
State	Published - Aug 5 2008

ASJC Scopus subject areas

General Chemistry

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10.1039/b803441a

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@article{d2afba5b592941cc944a13bea74105b2,

title = "The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex",

abstract = "The synthesis of [Ru(NO2)L(bpy)2]+ (bpy = 2,2′-bipyridine and L = pyridine (py) and pyrazine (pz)) can be accomplished by addition of [Ru(NO)L(bpy)2](PF6)3 to aqueous solutions of physiological pH. The electrochemical processes of [Ru(NO2)L(bpy)2]+ in aqueous solution were studied by cyclic voltammetry and differential pulse voltammetry. The anodic scan shows a peak around 1.00 V vs. Ag/AgCl attributed to the oxidation process centered on the metal ion. However, in the cathodic scan a second peak around −0.60 V vs. Ag/AgCl was observed and attributed to the reduction process centered on the nitrite ligand. The controlled reduction potential electrolysis at −0.80 V vs. Ag/AgCl shows NO release characteristics as judged by NO measurement with a NO-sensor. This assumption was confirmed by ESI/MS+ and spectroelectrochemical experiment where cis-[Ru(bpy)2L(H2O)]2+ was obtained as a product of the reduction of cis-[RuII(NO2)L(bpy)2]+. The vasorelaxation observed in denuded aortic rings pre-contracted with 0.1 μmol L−1 phenylephrine responded with relaxation in the presence of cis-[RuII(NO2)L(bpy)2]+. The potential of rat aorta cells to metabolize cis-[RuII(NO2)L(bpy)2]+ was also followed by confocal analysis. The obtained results suggest that NO release happens by reduction of cis-[RuII(NO2)L(bpy)2]+ inside the cell. The maximum vasorelaxation was achieved with 1 × 10−5 mol L−1 of cis-[RuII(NO2)L(bpy)2]+ complex.",

author = "\{Da Rocha\}, \{Z{\^e}nis Novais\} and Marchesi, \{Mario S{\'e}rgio Pereira\} and Molin, \{Josiane Cristina\} and Lunardi, \{Claure N.\} and Miranda, \{Katrina M.\} and Bendhack, \{Lusiane Maria\} and Ford, \{Peter C.\} and \{Da Silva\}, \{Roberto Santana\}",

year = "2008",

month = aug,

day = "5",

doi = "10.1039/b803441a",

language = "English (US)",

pages = "4282--4287",

journal = "Journal of the Chemical Society. Dalton Transactions",

issn = "1477-9226",

publisher = "Royal Society of Chemistry",

number = "32",

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TY - JOUR

T1 - The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO2)L(bpy)2]+ complex

AU - Da Rocha, Zênis Novais

AU - Marchesi, Mario Sérgio Pereira

AU - Molin, Josiane Cristina

AU - Lunardi, Claure N.

AU - Miranda, Katrina M.

AU - Bendhack, Lusiane Maria

AU - Ford, Peter C.

AU - Da Silva, Roberto Santana

PY - 2008/8/5

Y1 - 2008/8/5

N2 - The synthesis of [Ru(NO2)L(bpy)2]+ (bpy = 2,2′-bipyridine and L = pyridine (py) and pyrazine (pz)) can be accomplished by addition of [Ru(NO)L(bpy)2](PF6)3 to aqueous solutions of physiological pH. The electrochemical processes of [Ru(NO2)L(bpy)2]+ in aqueous solution were studied by cyclic voltammetry and differential pulse voltammetry. The anodic scan shows a peak around 1.00 V vs. Ag/AgCl attributed to the oxidation process centered on the metal ion. However, in the cathodic scan a second peak around −0.60 V vs. Ag/AgCl was observed and attributed to the reduction process centered on the nitrite ligand. The controlled reduction potential electrolysis at −0.80 V vs. Ag/AgCl shows NO release characteristics as judged by NO measurement with a NO-sensor. This assumption was confirmed by ESI/MS+ and spectroelectrochemical experiment where cis-[Ru(bpy)2L(H2O)]2+ was obtained as a product of the reduction of cis-[RuII(NO2)L(bpy)2]+. The vasorelaxation observed in denuded aortic rings pre-contracted with 0.1 μmol L−1 phenylephrine responded with relaxation in the presence of cis-[RuII(NO2)L(bpy)2]+. The potential of rat aorta cells to metabolize cis-[RuII(NO2)L(bpy)2]+ was also followed by confocal analysis. The obtained results suggest that NO release happens by reduction of cis-[RuII(NO2)L(bpy)2]+ inside the cell. The maximum vasorelaxation was achieved with 1 × 10−5 mol L−1 of cis-[RuII(NO2)L(bpy)2]+ complex.

AB - The synthesis of [Ru(NO2)L(bpy)2]+ (bpy = 2,2′-bipyridine and L = pyridine (py) and pyrazine (pz)) can be accomplished by addition of [Ru(NO)L(bpy)2](PF6)3 to aqueous solutions of physiological pH. The electrochemical processes of [Ru(NO2)L(bpy)2]+ in aqueous solution were studied by cyclic voltammetry and differential pulse voltammetry. The anodic scan shows a peak around 1.00 V vs. Ag/AgCl attributed to the oxidation process centered on the metal ion. However, in the cathodic scan a second peak around −0.60 V vs. Ag/AgCl was observed and attributed to the reduction process centered on the nitrite ligand. The controlled reduction potential electrolysis at −0.80 V vs. Ag/AgCl shows NO release characteristics as judged by NO measurement with a NO-sensor. This assumption was confirmed by ESI/MS+ and spectroelectrochemical experiment where cis-[Ru(bpy)2L(H2O)]2+ was obtained as a product of the reduction of cis-[RuII(NO2)L(bpy)2]+. The vasorelaxation observed in denuded aortic rings pre-contracted with 0.1 μmol L−1 phenylephrine responded with relaxation in the presence of cis-[RuII(NO2)L(bpy)2]+. The potential of rat aorta cells to metabolize cis-[RuII(NO2)L(bpy)2]+ was also followed by confocal analysis. The obtained results suggest that NO release happens by reduction of cis-[RuII(NO2)L(bpy)2]+ inside the cell. The maximum vasorelaxation was achieved with 1 × 10−5 mol L−1 of cis-[RuII(NO2)L(bpy)2]+ complex.

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DO - 10.1039/b803441a

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AN - SCOPUS:49149088638

SN - 1477-9226

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EP - 4287

JO - Journal of the Chemical Society. Dalton Transactions

JF - Journal of the Chemical Society. Dalton Transactions

IS - 32

ER -

The inducing NO-vasodilation by chemical reduction of coordinated nitrite ion in cis-[Ru(NO₂)L(bpy)₂]⁺ complex

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