The human PD-1 gene: Complete cDNA, genomic organization, and developmentally regulated expression in B cell progenitors

Lawrence R. Finger, Jaiyu Pu, Robert Wasserman, Rajeev Vibhakar, Elaine Louie, Richard R. Hardy, Peter D. Burrows, Linda G. Billips

Research output: Contribution to journalArticlepeer-review

92 Scopus citations

Abstract

We report the complete cDNA sequence and the genomic structure of the human PD-1 homologue. An analysis of the expression pattern of the human PD-1 gene (hPD-1) and the murine PD-1 gene (mPD-1) in developing bone marrow B-lineage cells was also undertaken. The full length hPD-1 cDNA is 2106 nucleotides long and encodes a predicted protein of 288 amino acid residues. The hPD-1 and mPD-1 genes share 70% homology at the nucleotide level and 60% homology at the amino acid level. Four potential sites for N-linked glycosylation are conserved, as are a stretch of amino acids between two cysteine residues resembling a V-set immunoglobulin domain, and another region containing a motif similar to an immunoreceptor tyrosine-based inhibitory motif. Isolation of the genomic locus of the hPD-1 gene reveals that the gene is composed of five exons located on human chromosome 2 at band q37. The 5' flanking region lacks TATA and CAAT cis-acting elements, but includes a number of potential transcription factor binding sites and a dominant transcription start site. The mPD-1 gene was preferentially expressed in pro-B cells from murine adult bone marrow. Although hPD-1 was not preferentially expressed in pro-B cells from human fetal bone marrow, treatment of isolated pro-B cells with interleukin-7 resulted in a dramatic increase in expression. These data suggest that PD-1 may play a role in B-cell differentiation during the pro-B cell stage.

Original languageEnglish (US)
Pages (from-to)177-187
Number of pages11
JournalGene
Volume197
Issue number1-2
DOIs
StatePublished - Sep 15 1997
Externally publishedYes

Keywords

  • DNA sequencing
  • Gene structure
  • Pro B cells

ASJC Scopus subject areas

  • Genetics

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