TY - JOUR
T1 - The herbicide sensitivity gene CHL1 of arabidopsis encodes a nitrate-inducible nitrate transporter
AU - Tsay, Yi Fang
AU - Schroeder, Julian I.
AU - Feldmann, Kenneth A.
AU - Crawford, Nigel M.
N1 - Funding Information:
We would like to thank Dr. M. Yanofsky and S. Kempin for help rn mapping the CHL7 clone by restriction fragment length polymorphism analysis, Y. Cao, Dr. M. Anderova, and Dr. D. Schachtman for their assistance in oocyte injection and recording; Dr. J. Wilkinson for his advice on plant transformation; Dr. Pablo Scolnik for sending mutant seed; and Dr. M. Saier for fruitful discussions. This work was supported by grants from the Powell Foundation and the National Institutes of Health (GM40672) to N. M. C. and in part by the National Science Foundation (DCB9004977) to J. I. S.
PY - 1993/3/12
Y1 - 1993/3/12
N2 - This paper reportsthe identification and functional expression of a gene that is involved in nitrate uptake in plants, a process essential for the assimilation of nitrate and the biological removal of nitrate from the soil solution. The CHL1 gene of Arabldopsis, which when mutated confers resistance to the herbicide chlorate and a decrease in nitrate uptake, was isolated and found to encode a protein with 12 putative membrane-spanning segments. Injection of CHL1 mRNA into Xenopus oocytes produces a nitrate- and pH-dependent membrane depolarization, inward current, and nitrate uptake. These data show that the CHL1 gene encodes an electrogenic nitrate transporter. CHL1 mRNA is found predominantly in roots and displays nitrate- and pH-dependent regulation.
AB - This paper reportsthe identification and functional expression of a gene that is involved in nitrate uptake in plants, a process essential for the assimilation of nitrate and the biological removal of nitrate from the soil solution. The CHL1 gene of Arabldopsis, which when mutated confers resistance to the herbicide chlorate and a decrease in nitrate uptake, was isolated and found to encode a protein with 12 putative membrane-spanning segments. Injection of CHL1 mRNA into Xenopus oocytes produces a nitrate- and pH-dependent membrane depolarization, inward current, and nitrate uptake. These data show that the CHL1 gene encodes an electrogenic nitrate transporter. CHL1 mRNA is found predominantly in roots and displays nitrate- and pH-dependent regulation.
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U2 - 10.1016/0092-8674(93)90399-B
DO - 10.1016/0092-8674(93)90399-B
M3 - Article
C2 - 8453665
AN - SCOPUS:0027526320
SN - 0092-8674
VL - 72
SP - 705
EP - 713
JO - Cell
JF - Cell
IS - 5
ER -