The Gαs-protein-mediated pathway may be steadily stimulated by prostanoid EP2 receptors, but not by EP4 receptors

Keijo Fukushima, Kanaho Senoo, Naoki Kurata, John W. Regan, Hiromichi Fujino

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

EP2 and EP4 prostanoid receptors have long been considered to have similar roles, since they are known to couple with Gαs-protein and activate cAMP-mediated signaling pathways. In this study, we re-evaluated the results of cAMP assays with or without phosphodiesterase (PDE) inhibitor pretreatment. Here, we show that in the absence of PDE inhibitor pretreatment, prostaglandin E2 causes accumulation of cAMP in EP2 receptors, whereas markedly low levels of cAMP accumulated in EP4 receptors. By applying the Black/Leff operational model calculation, we found that EP2 receptors have a biased ability to intrinsically activate the Gαs-protein-mediated pathway, whereas EP4 receptors have strong biased activity for the Gαi-protein-mediated pathway. Thus, EP2 and EP4 receptors may not be similar Gαs-coupled receptors but instead substantially different receptors with distinct roles.

Original languageEnglish (US)
Pages (from-to)775-783
Number of pages9
JournalFEBS Open Bio
Volume12
Issue number4
DOIs
StatePublished - Apr 2022

Keywords

  • PDE
  • biased signaling
  • black/Leff operational model calculation
  • cAMP
  • prostanoid EP2 receptor
  • prostanoid EP4 receptor

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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