The full-length clone of cucumber green mottle mosaic virus and its application as an expression system for Hepatitis B surface antigen

Aikseng Ooi; Sianghee Tan; Rosmawati Mohamed; Noorsaadah Abdul Rahman; Rofina Yasmin Othman

doi:10.1016/j.jbiotec.2005.08.032

The full-length clone of cucumber green mottle mosaic virus and its application as an expression system for Hepatitis B surface antigen

Aikseng Ooi, Sianghee Tan, Rosmawati Mohamed, Noorsaadah Abdul Rahman, Rofina Yasmin Othman

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

A cucumber green mosaic mottle virus (CGMMV) full-length clone was developed for the expression of Hepatitis B surface antigen (HBsAg). The expression of the surface displayed HBsAg by the chimeric virus was confirmed through a double antibody sandwich ELISA. Assessment of the coat protein composition of the chimeric virus particles by SDS-PAGE analysis showed that 50% of the coat proteins were fused to the HBsAg. Biological activity of the expressed HBsAg was assessed through the stimulation of in vitro antibody production by cultured peripheral blood mononuclear cells (PBMC). PBMC that were cultured in the presence of the chimeric virus showed up to an approximately three-fold increase in the level of anti HBsAg immunoglobulin thus suggesting the possible use of this new chimeric virus as an effective Hepatitis B vaccine.

Original language	English (US)
Pages (from-to)	471-481
Number of pages	11
Journal	Journal of Biotechnology
Volume	121
Issue number	4
DOIs	https://doi.org/10.1016/j.jbiotec.2005.08.032
State	Published - Feb 24 2006
Externally published	Yes

Keywords

CGMMV
Chimeric virus
HBsAg
Infectious transcripts
Tobamovirus

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiotec.2005.08.032

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title = "The full-length clone of cucumber green mottle mosaic virus and its application as an expression system for Hepatitis B surface antigen",

abstract = "A cucumber green mosaic mottle virus (CGMMV) full-length clone was developed for the expression of Hepatitis B surface antigen (HBsAg). The expression of the surface displayed HBsAg by the chimeric virus was confirmed through a double antibody sandwich ELISA. Assessment of the coat protein composition of the chimeric virus particles by SDS-PAGE analysis showed that 50% of the coat proteins were fused to the HBsAg. Biological activity of the expressed HBsAg was assessed through the stimulation of in vitro antibody production by cultured peripheral blood mononuclear cells (PBMC). PBMC that were cultured in the presence of the chimeric virus showed up to an approximately three-fold increase in the level of anti HBsAg immunoglobulin thus suggesting the possible use of this new chimeric virus as an effective Hepatitis B vaccine.",

keywords = "CGMMV, Chimeric virus, HBsAg, Infectious transcripts, Tobamovirus",

author = "Aikseng Ooi and Sianghee Tan and Rosmawati Mohamed and Rahman, {Noorsaadah Abdul} and Othman, {Rofina Yasmin}",

note = "Funding Information: This project is funded by Ministry of Science Technology and Innovation, Malaysia 36-02-03-6003 and National Science Fellowship (Malaysia). We would also like to thank Dr. Marry Anne Tan, Mr. Choy Mun Kit, Mr. Chai Jen Wai, and Mr. Ho Kian Kheong.",

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doi = "10.1016/j.jbiotec.2005.08.032",

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AU - Ooi, Aikseng

AU - Tan, Sianghee

AU - Mohamed, Rosmawati

AU - Rahman, Noorsaadah Abdul

AU - Othman, Rofina Yasmin

N1 - Funding Information: This project is funded by Ministry of Science Technology and Innovation, Malaysia 36-02-03-6003 and National Science Fellowship (Malaysia). We would also like to thank Dr. Marry Anne Tan, Mr. Choy Mun Kit, Mr. Chai Jen Wai, and Mr. Ho Kian Kheong.

PY - 2006/2/24

Y1 - 2006/2/24

N2 - A cucumber green mosaic mottle virus (CGMMV) full-length clone was developed for the expression of Hepatitis B surface antigen (HBsAg). The expression of the surface displayed HBsAg by the chimeric virus was confirmed through a double antibody sandwich ELISA. Assessment of the coat protein composition of the chimeric virus particles by SDS-PAGE analysis showed that 50% of the coat proteins were fused to the HBsAg. Biological activity of the expressed HBsAg was assessed through the stimulation of in vitro antibody production by cultured peripheral blood mononuclear cells (PBMC). PBMC that were cultured in the presence of the chimeric virus showed up to an approximately three-fold increase in the level of anti HBsAg immunoglobulin thus suggesting the possible use of this new chimeric virus as an effective Hepatitis B vaccine.

AB - A cucumber green mosaic mottle virus (CGMMV) full-length clone was developed for the expression of Hepatitis B surface antigen (HBsAg). The expression of the surface displayed HBsAg by the chimeric virus was confirmed through a double antibody sandwich ELISA. Assessment of the coat protein composition of the chimeric virus particles by SDS-PAGE analysis showed that 50% of the coat proteins were fused to the HBsAg. Biological activity of the expressed HBsAg was assessed through the stimulation of in vitro antibody production by cultured peripheral blood mononuclear cells (PBMC). PBMC that were cultured in the presence of the chimeric virus showed up to an approximately three-fold increase in the level of anti HBsAg immunoglobulin thus suggesting the possible use of this new chimeric virus as an effective Hepatitis B vaccine.

KW - CGMMV

KW - Chimeric virus

KW - HBsAg

KW - Infectious transcripts

KW - Tobamovirus

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The full-length clone of cucumber green mottle mosaic virus and its application as an expression system for Hepatitis B surface antigen

Abstract

Keywords

ASJC Scopus subject areas

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