The effects of dietary selenomethionine on polyamines and azoxymethane-induced aberrant crypts

Antonio T. Baines; Hanna Holubec; Jenny L. Basye; Patricia Thorne; Achyut K. Bhattacharyya; Julian Spallholz; Brent Shriver; Haiyan Cui; Denise Roe; Larry C. Clark; David L. Earnest; Mark A. Nelson

doi:10.1016/S0304-3835(00)00585-1

The effects of dietary selenomethionine on polyamines and azoxymethane-induced aberrant crypts

Antonio T. Baines, Hanna Holubec, Jenny L. Basye, Patricia Thorne, Achyut K. Bhattacharyya, Julian Spallholz, Brent Shriver, Haiyan Cui, Denise Roe, Larry C. Clark, David L. Earnest, Mark A. Nelson

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We evaluated the effects of dietary selenomethionine supplementation on colonic polyamine levels and the ability of l-selenomethionine supplementation to modulate the carcinogenic activity of azoxymethane (AOM) in the rat colon. Four-week-old male F344 rats were treated with 15 mg/kg body weight of AOM once a week for 2 weeks. Dietary selenomethionine at a concentration of either 1 or 2 ppm was administered in AIN-76A rodent diet to AOM-treated animals for 16 weeks. Aberrant crypt foci (ACF), precursor lesions of colon cancer, were investigated after the 16 week treatment course. Selenomethionine given in the diet at 2 ppm markedly reduced the number of aberrant crypt foci. The multiplicity of ACFs (i.e. the number of aberrant crypts/focus) and the percentage of microadenomas were also affected by selenomethionine in a dose dependent manner. However, evaluation of the colonic tissue polyamine levels between control and treated groups showed no significant difference. These results demonstrate that selenomethionine can modulate the development of AOM-induced premalignant lesions through a polyamine-independent mechanism. (C) 2000 Elsevier Science Ireland Ltd.

Original language	English (US)
Pages (from-to)	193-198
Number of pages	6
Journal	Cancer Letters
Volume	160
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3835(00)00585-1
State	Published - Nov 28 2000

Keywords

Abberant crypts
Cancer prevention
Selenomethionine

ASJC Scopus subject areas

Oncology
Cancer Research

Access to Document

10.1016/S0304-3835(00)00585-1

Cite this

@article{48f235bd38334c8fad03d550a3c427e4,

title = "The effects of dietary selenomethionine on polyamines and azoxymethane-induced aberrant crypts",

abstract = "We evaluated the effects of dietary selenomethionine supplementation on colonic polyamine levels and the ability of l-selenomethionine supplementation to modulate the carcinogenic activity of azoxymethane (AOM) in the rat colon. Four-week-old male F344 rats were treated with 15 mg/kg body weight of AOM once a week for 2 weeks. Dietary selenomethionine at a concentration of either 1 or 2 ppm was administered in AIN-76A rodent diet to AOM-treated animals for 16 weeks. Aberrant crypt foci (ACF), precursor lesions of colon cancer, were investigated after the 16 week treatment course. Selenomethionine given in the diet at 2 ppm markedly reduced the number of aberrant crypt foci. The multiplicity of ACFs (i.e. the number of aberrant crypts/focus) and the percentage of microadenomas were also affected by selenomethionine in a dose dependent manner. However, evaluation of the colonic tissue polyamine levels between control and treated groups showed no significant difference. These results demonstrate that selenomethionine can modulate the development of AOM-induced premalignant lesions through a polyamine-independent mechanism. (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "Abberant crypts, Cancer prevention, Selenomethionine",

author = "Baines, {Antonio T.} and Hanna Holubec and Basye, {Jenny L.} and Patricia Thorne and Bhattacharyya, {Achyut K.} and Julian Spallholz and Brent Shriver and Haiyan Cui and Denise Roe and Clark, {Larry C.} and Earnest, {David L.} and Nelson, {Mark A.}",

note = "Funding Information: We thank Dr Eugene Gerner and Mrs Sarah Marek for assistance with tissue polyamine analysis. We also thank Mr Frank Wamsley of the Southwest Environmental Health Science Center histopathology core service for tissue processing. This work was supported in part by Cypress Systems (M.A.N.), American Institute for Cancer Research (M.A.N.), CA 72008, ES 06694, ES 07091. In memory of our colleague Dr Larry Clark.",

year = "2000",

month = nov,

day = "28",

doi = "10.1016/S0304-3835(00)00585-1",

language = "English (US)",

volume = "160",

pages = "193--198",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - The effects of dietary selenomethionine on polyamines and azoxymethane-induced aberrant crypts

AU - Baines, Antonio T.

AU - Holubec, Hanna

AU - Basye, Jenny L.

AU - Thorne, Patricia

AU - Bhattacharyya, Achyut K.

AU - Spallholz, Julian

AU - Shriver, Brent

AU - Cui, Haiyan

AU - Roe, Denise

AU - Clark, Larry C.

AU - Earnest, David L.

AU - Nelson, Mark A.

N1 - Funding Information: We thank Dr Eugene Gerner and Mrs Sarah Marek for assistance with tissue polyamine analysis. We also thank Mr Frank Wamsley of the Southwest Environmental Health Science Center histopathology core service for tissue processing. This work was supported in part by Cypress Systems (M.A.N.), American Institute for Cancer Research (M.A.N.), CA 72008, ES 06694, ES 07091. In memory of our colleague Dr Larry Clark.

PY - 2000/11/28

Y1 - 2000/11/28

N2 - We evaluated the effects of dietary selenomethionine supplementation on colonic polyamine levels and the ability of l-selenomethionine supplementation to modulate the carcinogenic activity of azoxymethane (AOM) in the rat colon. Four-week-old male F344 rats were treated with 15 mg/kg body weight of AOM once a week for 2 weeks. Dietary selenomethionine at a concentration of either 1 or 2 ppm was administered in AIN-76A rodent diet to AOM-treated animals for 16 weeks. Aberrant crypt foci (ACF), precursor lesions of colon cancer, were investigated after the 16 week treatment course. Selenomethionine given in the diet at 2 ppm markedly reduced the number of aberrant crypt foci. The multiplicity of ACFs (i.e. the number of aberrant crypts/focus) and the percentage of microadenomas were also affected by selenomethionine in a dose dependent manner. However, evaluation of the colonic tissue polyamine levels between control and treated groups showed no significant difference. These results demonstrate that selenomethionine can modulate the development of AOM-induced premalignant lesions through a polyamine-independent mechanism. (C) 2000 Elsevier Science Ireland Ltd.

AB - We evaluated the effects of dietary selenomethionine supplementation on colonic polyamine levels and the ability of l-selenomethionine supplementation to modulate the carcinogenic activity of azoxymethane (AOM) in the rat colon. Four-week-old male F344 rats were treated with 15 mg/kg body weight of AOM once a week for 2 weeks. Dietary selenomethionine at a concentration of either 1 or 2 ppm was administered in AIN-76A rodent diet to AOM-treated animals for 16 weeks. Aberrant crypt foci (ACF), precursor lesions of colon cancer, were investigated after the 16 week treatment course. Selenomethionine given in the diet at 2 ppm markedly reduced the number of aberrant crypt foci. The multiplicity of ACFs (i.e. the number of aberrant crypts/focus) and the percentage of microadenomas were also affected by selenomethionine in a dose dependent manner. However, evaluation of the colonic tissue polyamine levels between control and treated groups showed no significant difference. These results demonstrate that selenomethionine can modulate the development of AOM-induced premalignant lesions through a polyamine-independent mechanism. (C) 2000 Elsevier Science Ireland Ltd.

KW - Abberant crypts

KW - Cancer prevention

KW - Selenomethionine

UR - http://www.scopus.com/inward/record.url?scp=0034727780&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034727780&partnerID=8YFLogxK

U2 - 10.1016/S0304-3835(00)00585-1

DO - 10.1016/S0304-3835(00)00585-1

M3 - Article

C2 - 11053649

AN - SCOPUS:0034727780

SN - 0304-3835

VL - 160

SP - 193

EP - 198

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

The effects of dietary selenomethionine on polyamines and azoxymethane-induced aberrant crypts

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this