TY - JOUR
T1 - The effects of bFGF, IGF-I, and TGF-β on RMo skeletal muscle cell proliferation and differentiation
AU - Johnson, Sally E.
AU - Allen, Ronald E.
N1 - Funding Information:
This work was supported by the Arizona Agriculture Experiment Station, Project No. Rll, USPHS Grant AG-03393, and USDA Grants 86.CRCR-l-2023 and 89-37265-4474. This communication is Arizona Agriculture Experiment Station Publication No. 7146.
PY - 1990/4
Y1 - 1990/4
N2 - A new skeletal muscle cell line, rat myoblast ω or RMo, has been characterized with regard to the effects of three growth factors: basic fibroblast growth factor (bFGF), insulin-like growth factor I (IGF-I), and transforming growth factor β (TGF-β). Results indicate a differential response of these factors on both cell proliferation and differentiation. Exposure to bFGF and IGF-I stimulate proliferation, while TGF-β has no effect on cell number. RMo cell differentiation, as indicated by skeletal myosin synthesis, is enhanced by IGF-I, whereas both bFGF and TGF-β suppress differentiation. These responses are in agreement with the effects of bFGF, IGF-I, and TGF-β on myogenic cells cultured from fetal and postnatal muscle, thereby suggesting that RMo cells can serve as a model system for the study of growth factor effects on skeletal muscle cells.
AB - A new skeletal muscle cell line, rat myoblast ω or RMo, has been characterized with regard to the effects of three growth factors: basic fibroblast growth factor (bFGF), insulin-like growth factor I (IGF-I), and transforming growth factor β (TGF-β). Results indicate a differential response of these factors on both cell proliferation and differentiation. Exposure to bFGF and IGF-I stimulate proliferation, while TGF-β has no effect on cell number. RMo cell differentiation, as indicated by skeletal myosin synthesis, is enhanced by IGF-I, whereas both bFGF and TGF-β suppress differentiation. These responses are in agreement with the effects of bFGF, IGF-I, and TGF-β on myogenic cells cultured from fetal and postnatal muscle, thereby suggesting that RMo cells can serve as a model system for the study of growth factor effects on skeletal muscle cells.
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U2 - 10.1016/0014-4827(90)90088-R
DO - 10.1016/0014-4827(90)90088-R
M3 - Article
C2 - 2180733
AN - SCOPUS:0025270823
SN - 0014-4827
VL - 187
SP - 250
EP - 254
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -