The effects of ascorbic acid on dopamine-induced death of PC12 cells are dependent on exposure kinetics

Hong Y. Choi; Jin H. Song; Dong K. Park; Gregory M. Ross

doi:10.1016/S0304-3940(00)01647-5

The effects of ascorbic acid on dopamine-induced death of PC12 cells are dependent on exposure kinetics

Hong Y. Choi, Jin H. Song, Dong K. Park, Gregory M. Ross

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The role of ascorbic acid on dopamine (DA) oxidation-mediated cytotoxicity was studied using the PC12 cell line. DA cytotoxicity was slightly attenuated by ascorbic acid, whereas the cytotoxicity of 6-hydroxydopamine (6-OHDA), a DA oxidation product, was markedly potentiated. To elucidate the relationship between the ascorbic acid effect and the degree of DA oxidation, ascorbic acid was added in a time-dependent fashion after DA treatment. We found greater cell death the later ascorbic acid was applied. Treatment of cells with glutathione alleviated DA- and 6-OHDA-induced cell death, while L-buthionine sulfoximine potentiated DA and 6-OHDA cytotoxicity. Ascorbic acid combined with glutathione eliminated the toxicity of DA and 6-OHDA. These results suggest that the interaction between DA and ascorbic acid is dependent upon the degree of DA oxidation and glutathione availability. (C) 2000 Elsevier Science Ireland Ltd.

Original language	English (US)
Pages (from-to)	81-84
Number of pages	4
Journal	Neuroscience Letters
Volume	296
Issue number	2-3
DOIs	https://doi.org/10.1016/S0304-3940(00)01647-5
State	Published - Dec 22 2000
Externally published	Yes

Keywords

Catecholamines
Glutathione
L-Buthionine sulfoximine
Oxidative stress
Parkinson's disease

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/S0304-3940(00)01647-5

Cite this

@article{4082b4aecae245e5990fb42a50f31a63,

title = "The effects of ascorbic acid on dopamine-induced death of PC12 cells are dependent on exposure kinetics",

abstract = "The role of ascorbic acid on dopamine (DA) oxidation-mediated cytotoxicity was studied using the PC12 cell line. DA cytotoxicity was slightly attenuated by ascorbic acid, whereas the cytotoxicity of 6-hydroxydopamine (6-OHDA), a DA oxidation product, was markedly potentiated. To elucidate the relationship between the ascorbic acid effect and the degree of DA oxidation, ascorbic acid was added in a time-dependent fashion after DA treatment. We found greater cell death the later ascorbic acid was applied. Treatment of cells with glutathione alleviated DA- and 6-OHDA-induced cell death, while L-buthionine sulfoximine potentiated DA and 6-OHDA cytotoxicity. Ascorbic acid combined with glutathione eliminated the toxicity of DA and 6-OHDA. These results suggest that the interaction between DA and ascorbic acid is dependent upon the degree of DA oxidation and glutathione availability. (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "Catecholamines, Glutathione, L-Buthionine sulfoximine, Oxidative stress, Parkinson's disease",

author = "Choi, {Hong Y.} and Song, {Jin H.} and Park, {Dong K.} and Ross, {Gregory M.}",

note = "Funding Information: This work was supported by a grant from the Heart and Stroke Foundation of Ontario (NA3718) and the Canadian Institute of Health Research. GMR is a Queen's National Scholar.",

year = "2000",

month = dec,

day = "22",

doi = "10.1016/S0304-3940(00)01647-5",

language = "English (US)",

volume = "296",

pages = "81--84",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "2-3",

}

TY - JOUR

T1 - The effects of ascorbic acid on dopamine-induced death of PC12 cells are dependent on exposure kinetics

AU - Choi, Hong Y.

AU - Song, Jin H.

AU - Park, Dong K.

AU - Ross, Gregory M.

N1 - Funding Information: This work was supported by a grant from the Heart and Stroke Foundation of Ontario (NA3718) and the Canadian Institute of Health Research. GMR is a Queen's National Scholar.

PY - 2000/12/22

Y1 - 2000/12/22

N2 - The role of ascorbic acid on dopamine (DA) oxidation-mediated cytotoxicity was studied using the PC12 cell line. DA cytotoxicity was slightly attenuated by ascorbic acid, whereas the cytotoxicity of 6-hydroxydopamine (6-OHDA), a DA oxidation product, was markedly potentiated. To elucidate the relationship between the ascorbic acid effect and the degree of DA oxidation, ascorbic acid was added in a time-dependent fashion after DA treatment. We found greater cell death the later ascorbic acid was applied. Treatment of cells with glutathione alleviated DA- and 6-OHDA-induced cell death, while L-buthionine sulfoximine potentiated DA and 6-OHDA cytotoxicity. Ascorbic acid combined with glutathione eliminated the toxicity of DA and 6-OHDA. These results suggest that the interaction between DA and ascorbic acid is dependent upon the degree of DA oxidation and glutathione availability. (C) 2000 Elsevier Science Ireland Ltd.

AB - The role of ascorbic acid on dopamine (DA) oxidation-mediated cytotoxicity was studied using the PC12 cell line. DA cytotoxicity was slightly attenuated by ascorbic acid, whereas the cytotoxicity of 6-hydroxydopamine (6-OHDA), a DA oxidation product, was markedly potentiated. To elucidate the relationship between the ascorbic acid effect and the degree of DA oxidation, ascorbic acid was added in a time-dependent fashion after DA treatment. We found greater cell death the later ascorbic acid was applied. Treatment of cells with glutathione alleviated DA- and 6-OHDA-induced cell death, while L-buthionine sulfoximine potentiated DA and 6-OHDA cytotoxicity. Ascorbic acid combined with glutathione eliminated the toxicity of DA and 6-OHDA. These results suggest that the interaction between DA and ascorbic acid is dependent upon the degree of DA oxidation and glutathione availability. (C) 2000 Elsevier Science Ireland Ltd.

KW - Catecholamines

KW - Glutathione

KW - L-Buthionine sulfoximine

KW - Oxidative stress

KW - Parkinson's disease

UR - http://www.scopus.com/inward/record.url?scp=0034704381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034704381&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(00)01647-5

DO - 10.1016/S0304-3940(00)01647-5

M3 - Article

C2 - 11108986

AN - SCOPUS:0034704381

SN - 0304-3940

VL - 296

SP - 81

EP - 84

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2-3

ER -

The effects of ascorbic acid on dopamine-induced death of PC12 cells are dependent on exposure kinetics

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this