The effects of ascorbic acid on dopamine-induced death of PC12 cells are dependent on exposure kinetics

Hong Y. Choi, Jin H. Song, Dong K. Park, Gregory M. Ross

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The role of ascorbic acid on dopamine (DA) oxidation-mediated cytotoxicity was studied using the PC12 cell line. DA cytotoxicity was slightly attenuated by ascorbic acid, whereas the cytotoxicity of 6-hydroxydopamine (6-OHDA), a DA oxidation product, was markedly potentiated. To elucidate the relationship between the ascorbic acid effect and the degree of DA oxidation, ascorbic acid was added in a time-dependent fashion after DA treatment. We found greater cell death the later ascorbic acid was applied. Treatment of cells with glutathione alleviated DA- and 6-OHDA-induced cell death, while L-buthionine sulfoximine potentiated DA and 6-OHDA cytotoxicity. Ascorbic acid combined with glutathione eliminated the toxicity of DA and 6-OHDA. These results suggest that the interaction between DA and ascorbic acid is dependent upon the degree of DA oxidation and glutathione availability. (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalNeuroscience Letters
Volume296
Issue number2-3
DOIs
StatePublished - Dec 22 2000
Externally publishedYes

Keywords

  • Catecholamines
  • Glutathione
  • L-Buthionine sulfoximine
  • Oxidative stress
  • Parkinson's disease

ASJC Scopus subject areas

  • Neuroscience(all)

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