The effect of choline deficiency on the activity of a phosphatidylcholine-requiring enzyme: Activity and properties of UDP-glucuronyltransferase in choline-deficient rats

Thomas David Boyer; David Zakim

doi:10.1016/0006-291X(83)91643-1

The effect of choline deficiency on the activity of a phosphatidylcholine-requiring enzyme: Activity and properties of UDP-glucuronyltransferase in choline-deficient rats

Thomas David Boyer, David Zakim

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7 Scopus citations

Abstract

The effect of choline deficiency on the kinetic properties of the microsomal enzyme UDP-glucuronyltransferase (EC2.4.1.17) was investigated in rats. Animals fed choline-deficient diets, as compared with animals fed a choline-replete diet or standard laboratory chow, showed almost a three-fold increase in enzyme activity when the enzyme was assayed at physiological concentrations of UDP-glucuronic acid (0.25 mM). The increase in activity appeared to be due to an enhanced affinity of the enzyme for UDP-glucuronic acid rather than to an increase in the amount of enzyme. These data indicate that the kinetic properties of tightly bound membrane enzymes are altered by a dietary change that is known to cause liver disease in the rat.

Original language	English (US)
Pages (from-to)	418-424
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	114
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(83)91643-1
State	Published - Jul 18 1983
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(83)91643-1

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The effect of choline deficiency on the activity of a phosphatidylcholine-requiring enzyme: Activity and properties of UDP-glucuronyltransferase in choline-deficient rats. / Boyer, Thomas David; Zakim, David.
In: Biochemical and Biophysical Research Communications, Vol. 114, No. 1, 18.07.1983, p. 418-424.

Research output: Contribution to journal › Article › peer-review

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abstract = "The effect of choline deficiency on the kinetic properties of the microsomal enzyme UDP-glucuronyltransferase (EC2.4.1.17) was investigated in rats. Animals fed choline-deficient diets, as compared with animals fed a choline-replete diet or standard laboratory chow, showed almost a three-fold increase in enzyme activity when the enzyme was assayed at physiological concentrations of UDP-glucuronic acid (0.25 mM). The increase in activity appeared to be due to an enhanced affinity of the enzyme for UDP-glucuronic acid rather than to an increase in the amount of enzyme. These data indicate that the kinetic properties of tightly bound membrane enzymes are altered by a dietary change that is known to cause liver disease in the rat.",

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The effect of choline deficiency on the activity of a phosphatidylcholine-requiring enzyme: Activity and properties of UDP-glucuronyltransferase in choline-deficient rats

Abstract

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