The E295K DNA polymerase beta gastric cancer-associated variant interferes with base excision repair and induces cellular transformation

Tieming Lang, Shibani Dalal, Anna Chikova, Daniel DiMaio, Joann B. Sweasy

Research output: Contribution to journalArticlepeer-review

84 Scopus citations

Abstract

Approximately 30% of human tumors examined for mutations in polymerase beta (pol β) appear to express pol β variant proteins (D. Starcevic, S. Dalal, and J. B. Sweasy, Cell Cycle 3:998-1001, 2004). Many of these variants result from a single amino acid substitution. We have previously shown that the K289M and I260M colon and prostate cancer variants, respectively, induce cellular transformation most likely due to sequence-specific mutator activity (S. Dalal et al., Biochemistry 44:15664-15673, 2005; T. Lang et al., Proc. Natl. Acad. Sci. USA 101:6074-6079, 2004; J. B. Sweasy et al., Proc. Natl. Acad. Sci. USA 102:14350-14355, 2005). In the work described here, we show that the E295K gastric carcinoma pol β variant acts in a dominant-negative manner by interfering with base excision repair. This leads to an increase in sister chromatid exchanges. Expression of the E295K variant also induces cellular transformation. Our data suggest that unfilled gaps are channeled into a homology-directed repair pathway that could lead to genomic instability. The results indicate that base excision repair is critical for maintaining genome stability and could therefore be a tumor suppressor mechanism.

Original languageEnglish (US)
Pages (from-to)5587-5596
Number of pages10
JournalMolecular and cellular biology
Volume27
Issue number15
DOIs
StatePublished - Aug 2007
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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