The distribution of la antigens of the H-2 complex on lymph node cells by immunoferritin labelling

Jane S. Schultz; Jeffrey A. Frelinger; Sun Kee Kim; Donald C. Shreffler

doi:10.1016/0008-8749(75)90191-4

The distribution of la antigens of the H-2 complex on lymph node cells by immunoferritin labelling

Jane S. Schultz, Jeffrey A. Frelinger, Sun Kee Kim, Donald C. Shreffler

Immunobiology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The distribution of Ia antigens on the surfaces of lymph node lymphocytes of several mouse strains was investigated using indirect immunoferritin labeling and electron microscopy. The immunoferritin labeling results agreed with results of cytotoxic tests in strain distribution of reactivity, proportion of cells showing label, and cell populations reacting. Capping was induced by increased incubation temperature but conditions for Ia antigen mobilization varied somewhat between the two anti-Ia antisera employed. Uncapped specimens generally showed a denser, more evenly distributed antigen coating than is the case for H-2 antigens labeled by the same indirect immunoferritin method.

Original language	English (US)
Pages (from-to)	125-134
Number of pages	10
Journal	Cellular Immunology
Volume	16
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(75)90191-4
State	Published - Mar 1975

ASJC Scopus subject areas

Immunology

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10.1016/0008-8749(75)90191-4

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@article{e79298a460544344b61f02446e4c5e3b,

title = "The distribution of la antigens of the H-2 complex on lymph node cells by immunoferritin labelling",

abstract = "The distribution of Ia antigens on the surfaces of lymph node lymphocytes of several mouse strains was investigated using indirect immunoferritin labeling and electron microscopy. The immunoferritin labeling results agreed with results of cytotoxic tests in strain distribution of reactivity, proportion of cells showing label, and cell populations reacting. Capping was induced by increased incubation temperature but conditions for Ia antigen mobilization varied somewhat between the two anti-Ia antisera employed. Uncapped specimens generally showed a denser, more evenly distributed antigen coating than is the case for H-2 antigens labeled by the same indirect immunoferritin method.",

author = "Schultz, {Jane S.} and Frelinger, {Jeffrey A.} and Kim, {Sun Kee} and Shreffler, {Donald C.}",

note = "Funding Information: The authors wish to acknowledge the excellent technical assistance of Virginia Hartog, and to thank Dr. Chella David for the two anti-Ia sera used. We would like, also, to thank Karen Dean for typing the manuscript. These are Veterans Administration Projects 7751: 01 and 4927: 01. This work was partially supported by USPHS Program Project Grant GM-15419 and USPHS Research Grant ROl-AI-11962. One of t+e authors (J.A.F.) is a Jane Coffin Childs{\textquoteright} Fellow, and another (D.C.S.) is the holder of USPHS Research Career Development Award K3-HL-24980.",

year = "1975",

month = mar,

doi = "10.1016/0008-8749(75)90191-4",

language = "English (US)",

volume = "16",

pages = "125--134",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press Inc.",

number = "1",

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T1 - The distribution of la antigens of the H-2 complex on lymph node cells by immunoferritin labelling

AU - Schultz, Jane S.

AU - Frelinger, Jeffrey A.

AU - Kim, Sun Kee

AU - Shreffler, Donald C.

N1 - Funding Information: The authors wish to acknowledge the excellent technical assistance of Virginia Hartog, and to thank Dr. Chella David for the two anti-Ia sera used. We would like, also, to thank Karen Dean for typing the manuscript. These are Veterans Administration Projects 7751: 01 and 4927: 01. This work was partially supported by USPHS Program Project Grant GM-15419 and USPHS Research Grant ROl-AI-11962. One of t+e authors (J.A.F.) is a Jane Coffin Childs’ Fellow, and another (D.C.S.) is the holder of USPHS Research Career Development Award K3-HL-24980.

PY - 1975/3

Y1 - 1975/3

N2 - The distribution of Ia antigens on the surfaces of lymph node lymphocytes of several mouse strains was investigated using indirect immunoferritin labeling and electron microscopy. The immunoferritin labeling results agreed with results of cytotoxic tests in strain distribution of reactivity, proportion of cells showing label, and cell populations reacting. Capping was induced by increased incubation temperature but conditions for Ia antigen mobilization varied somewhat between the two anti-Ia antisera employed. Uncapped specimens generally showed a denser, more evenly distributed antigen coating than is the case for H-2 antigens labeled by the same indirect immunoferritin method.

AB - The distribution of Ia antigens on the surfaces of lymph node lymphocytes of several mouse strains was investigated using indirect immunoferritin labeling and electron microscopy. The immunoferritin labeling results agreed with results of cytotoxic tests in strain distribution of reactivity, proportion of cells showing label, and cell populations reacting. Capping was induced by increased incubation temperature but conditions for Ia antigen mobilization varied somewhat between the two anti-Ia antisera employed. Uncapped specimens generally showed a denser, more evenly distributed antigen coating than is the case for H-2 antigens labeled by the same indirect immunoferritin method.

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JO - Cellular Immunology

JF - Cellular Immunology

IS - 1

ER -

The distribution of la antigens of the H-2 complex on lymph node cells by immunoferritin labelling

Abstract

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