The contribution of activated factor XIII to fibrinolytic resistance in experimental pulmonary embolism

Guy L. Reed, Aiilyan K. Houng

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

Background - The resistance of thrombi to fibrinolysis induced by plasminogen activators remains a major impediment to the successful treatment of thrombotic diseases. This study examines the contribution of activated factor XIII (factor XIIIa) to fibrinolytic resistance in experimental pulmonary embolism. Methods and Results - The fibrinolytic effects of specific inhibitors of factor XIIIa-mediated fibrin-fibrin cross-linking and α2-antiplasmin-fibrin cross-linking were measured in anesthetized ferrets with pulmonary emboli. Five experimental groups were treated with heparin (100 U/kg) and/or tissue plasminogen activator (TPA, 1 mg/kg) and the percent (mean ± SD) lysis of emboli was determined: (1) control, normal factor XIIIa activity (14.1 ± 4.8% lysis); (2) inhibited factor XIIIa activity (42.7 ± 7.4%); (3) normal factor XIIIa activity+TPA (32.3 ± 7.7%); (4) inhibited factor XIIIa activity+TPA (76.0 ± 11.9%); and (5) inhibited α2- antiplasmin-fibrin cross-linking+TPA (54.7 ± 3.9%). Inhibition of factor XIIIa activity increased endogenous lysis markedly (group 1 versus 2; P < 0.0001), to a level comparable to that achieved with TPA (group 2 versus 3; P < 0.05). Among groups receiving TPA, selective inhibition of factor XIII- mediated α2-antiplasmin-fibrin cross-linking enhanced lysis (group 3 versus 5; P < 0.0005). Complete inhibition of factor XIIIa also amplified lysis (group 3 versus 4; P < 0.0001) and had greater effects than inhibition of α2-antiplasmin cross-linking alone (group 4 versus 5; P < 0.0005). No significant fibrinogen degradation occurred in any group. Conclusions - Factor XIIIa-mediated fibrin-fibrin and α2-antiplasmin-fibrin cross-linking both caused experimental pulmonary emboli to resist endogenous and TPA- induced fibrinolysis. This suggests that factor XIIIa may play a critical role in regulating fibrinolysis in human thrombosis.

Original languageEnglish (US)
Pages (from-to)299-304
Number of pages6
JournalCirculation
Volume99
Issue number2
DOIs
StatePublished - Jan 19 1999
Externally publishedYes

Keywords

  • Embolism
  • Fibrinolysis
  • Plasminogen activators
  • Thrombus

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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