TY - JOUR
T1 - The complete nucleotide sequence and genome organization of red clover necrotic mosaic virus RNA-1
AU - Xiong, Z.
AU - Lommel, S. A.
N1 - Funding Information:
This research was supported in part by grants fromt he USDA (85-CRCR-l-1798 and 86-CRCR-1-2253) and an award from The McKnight Foundation. The authors thank Dr. L. Calvert, M. Weston-Fina, and Marietta Ryba-White for aid with the computer graphics and sequence analysis, and Dr. George Lomonossoff for generation and interpretation of the wandering spot data. This is Paper No. 12037 of the Journal Series of the North Carolina Agricultural Research Service, North Carolina State University, Raleigh, North Carolina 27695-7643.
PY - 1989/8
Y1 - 1989/8
N2 - The complete nucleotide sequence of red clover necrotic mosaic virus (RCNMV) RNA-1 has been determined. RNA-1 is 3889 nucleotides in length with a 5′ terminal m7GpppA cap. The RNA contains three large open reading frames (ORFs): the 5′ proximal ORF, encoding a 27-kDa polypeptide; the internal ORF, coding for a 57-kDa polypeptide; and the 3′ terminal ORF, encoding the 37-kDa capsid protein. The sequence results confirm in vitro translation of 27-, 50-, and 37-kDa products but do not account for the observed 90-kDa product. A translational frameshift event from the 27- to the 57-kDa ORFs is proposed to explain the synthesis of the observed 90-kDa in vitro product. The putative translational frameshift region is structurally similar to several retrovirus frameshift regions and the putative barley yellow dwarf virus (BYDV) frameshift region. Extensive amino acid homology was observed in the 57-kDa downstream ORF with the downstream domains of the carnation mottle virus (CarMV), turnip crinkle virus (TCV), maize chlorotic mottle virus (MCMV) readthrough, and BYDV fusion proteins. The 57-kDa ORF contained the conserved "GDD" motif. A significant alignment between the capsid proteins of RCNMV, CarMV, and TCV was also observed. Given the extensive amino acid sequence similarity of RCNMV, CarMV, and TCV polymerase and capsid proteins, we speculate that they are closely related, evolutionarily.
AB - The complete nucleotide sequence of red clover necrotic mosaic virus (RCNMV) RNA-1 has been determined. RNA-1 is 3889 nucleotides in length with a 5′ terminal m7GpppA cap. The RNA contains three large open reading frames (ORFs): the 5′ proximal ORF, encoding a 27-kDa polypeptide; the internal ORF, coding for a 57-kDa polypeptide; and the 3′ terminal ORF, encoding the 37-kDa capsid protein. The sequence results confirm in vitro translation of 27-, 50-, and 37-kDa products but do not account for the observed 90-kDa product. A translational frameshift event from the 27- to the 57-kDa ORFs is proposed to explain the synthesis of the observed 90-kDa in vitro product. The putative translational frameshift region is structurally similar to several retrovirus frameshift regions and the putative barley yellow dwarf virus (BYDV) frameshift region. Extensive amino acid homology was observed in the 57-kDa downstream ORF with the downstream domains of the carnation mottle virus (CarMV), turnip crinkle virus (TCV), maize chlorotic mottle virus (MCMV) readthrough, and BYDV fusion proteins. The 57-kDa ORF contained the conserved "GDD" motif. A significant alignment between the capsid proteins of RCNMV, CarMV, and TCV was also observed. Given the extensive amino acid sequence similarity of RCNMV, CarMV, and TCV polymerase and capsid proteins, we speculate that they are closely related, evolutionarily.
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U2 - 10.1016/0042-6822(89)90624-7
DO - 10.1016/0042-6822(89)90624-7
M3 - Article
C2 - 2763465
AN - SCOPUS:0024712866
SN - 0042-6822
VL - 171
SP - 543
EP - 554
JO - Virology
JF - Virology
IS - 2
ER -