The cloning and sequence analysis of the rat serotonin-1A receptor gene

Yutaka Fujiwara; David L. Nelson; Kenichi Kashihara; Eva Varga; William R. Roeske; Henry I. Yamamura

doi:10.1016/0024-3205(90)90445-W

The cloning and sequence analysis of the rat serotonin-1A receptor gene

Yutaka Fujiwara, David L. Nelson, Kenichi Kashihara, Eva Varga, William R. Roeske, Henry I. Yamamura

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Abstract

Using a probe derived from the human 5-HT_1A receptor gene (G-21), a strongly hybridizable clone was identified from a rat genomic DNA library. This clone (G21Y2) had an overall deduced amino acid sequence that was 88% homologous with that of the human 5-HT_1A receptor. There was only an 18% and 19% amino acid homology with the rat 5-HT_1C and 5-HT₂ receptors, respectively. The G21Y2 appears to be an excellent candidate for the rat 5-HT_1A receptor gene. As this manuscript was being prepared a report by Albert et al. (13) was published in which a probe to the hamster β₂-adrenergic receptor was used to identify a rat genomic (Clone D) that demonstrated a sequence that was similar to G21Y2.

Original language	English (US)
Pages (from-to)	PL127-PL132
Journal	Life Sciences
Volume	47
Issue number	22
DOIs	https://doi.org/10.1016/0024-3205(90)90445-W
State	Published - 1990

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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10.1016/0024-3205(90)90445-W

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title = "The cloning and sequence analysis of the rat serotonin-1A receptor gene",

abstract = "Using a probe derived from the human 5-HT1A receptor gene (G-21), a strongly hybridizable clone was identified from a rat genomic DNA library. This clone (G21Y2) had an overall deduced amino acid sequence that was 88% homologous with that of the human 5-HT1A receptor. There was only an 18% and 19% amino acid homology with the rat 5-HT1C and 5-HT2 receptors, respectively. The G21Y2 appears to be an excellent candidate for the rat 5-HT1A receptor gene. As this manuscript was being prepared a report by Albert et al. (13) was published in which a probe to the hamster β2-adrenergic receptor was used to identify a rat genomic (Clone D) that demonstrated a sequence that was similar to G21Y2.",

author = "Yutaka Fujiwara and Nelson, {David L.} and Kenichi Kashihara and Eva Varga and Roeske, {William R.} and Yamamura, {Henry I.}",

note = "Funding Information: We wish to thank Drs. Brian K. Kobilka, Marc Caron, and Robert J. Lefkowitz for their kind gift of the genomic clone (G-21) of the human 5-HTIA receptor gene and Dr. Josephine Lai for her discussions. This work was supported by NIMH grant MH-39530 and NIH grants NS-01009, HL-20984, DK-36289 and a grant from the Arizona Disease Control Research Commission.",

year = "1990",

doi = "10.1016/0024-3205(90)90445-W",

language = "English (US)",

volume = "47",

pages = "PL127--PL132",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

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T1 - The cloning and sequence analysis of the rat serotonin-1A receptor gene

AU - Fujiwara, Yutaka

AU - Nelson, David L.

AU - Kashihara, Kenichi

AU - Varga, Eva

AU - Roeske, William R.

AU - Yamamura, Henry I.

N1 - Funding Information: We wish to thank Drs. Brian K. Kobilka, Marc Caron, and Robert J. Lefkowitz for their kind gift of the genomic clone (G-21) of the human 5-HTIA receptor gene and Dr. Josephine Lai for her discussions. This work was supported by NIMH grant MH-39530 and NIH grants NS-01009, HL-20984, DK-36289 and a grant from the Arizona Disease Control Research Commission.

PY - 1990

Y1 - 1990

N2 - Using a probe derived from the human 5-HT1A receptor gene (G-21), a strongly hybridizable clone was identified from a rat genomic DNA library. This clone (G21Y2) had an overall deduced amino acid sequence that was 88% homologous with that of the human 5-HT1A receptor. There was only an 18% and 19% amino acid homology with the rat 5-HT1C and 5-HT2 receptors, respectively. The G21Y2 appears to be an excellent candidate for the rat 5-HT1A receptor gene. As this manuscript was being prepared a report by Albert et al. (13) was published in which a probe to the hamster β2-adrenergic receptor was used to identify a rat genomic (Clone D) that demonstrated a sequence that was similar to G21Y2.

AB - Using a probe derived from the human 5-HT1A receptor gene (G-21), a strongly hybridizable clone was identified from a rat genomic DNA library. This clone (G21Y2) had an overall deduced amino acid sequence that was 88% homologous with that of the human 5-HT1A receptor. There was only an 18% and 19% amino acid homology with the rat 5-HT1C and 5-HT2 receptors, respectively. The G21Y2 appears to be an excellent candidate for the rat 5-HT1A receptor gene. As this manuscript was being prepared a report by Albert et al. (13) was published in which a probe to the hamster β2-adrenergic receptor was used to identify a rat genomic (Clone D) that demonstrated a sequence that was similar to G21Y2.

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JO - Life Sciences

JF - Life Sciences

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ER -

The cloning and sequence analysis of the rat serotonin-1A receptor gene

Abstract

ASJC Scopus subject areas

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