The cloning and expression of a human creatine transporter

Ichiro Sora; Jeremy Richman; Giovanna Santoro; Hongbing Wei; Yu Wang; Todd Vanderah; Robert Horvath; Mike Nguyen; Sue Waite; William R. Roeske; Henry I. Yamamura

doi:10.1006/bbrc.1994.2475

The cloning and expression of a human creatine transporter

Ichiro Sora
, Jeremy Richman
, Giovanna Santoro
, Hongbing Wei
, Yu Wang
, Todd Vanderah
, Robert Horvath
, Mike Nguyen
, Sue Waite
, William R. Roeske
, Henry I. Yamamura

Research output: Contribution to journal › Article › peer-review

117 Scopus citations

Abstract

A human creatine transporter (hCRT-BS2M) cDNA clone was isolated from a human brainstem/spinal cord using a PCR and phage plaque hybridization based technique. This clone included an open reading frame of 1,905 base pairs(bp) within a 2,283bp cDNA. Northern blot hybridization detected the expression of corresponding mRNAs most prominently in the skeletal muscle, heart and kidney. Peptide sequence analysis of the hCRT-BS2M protein product revealed 12 putative transmembrane domains. The predicted protein sequence further demonstrates that the hCRT-BS2M has highly conserved amino acid identity with the other members of the sodium dependent plasma membrane transporter family. Transient expression of the hCRT-BS2M in COS-7 cells demonstrates sodium dependent [¹⁴C]creatine uptake with a K_M value of 14.9 ± 3.0 μM (n=5) that is attenuated by creatine and selective structural analogues of creatine.

Original language	English (US)
Pages (from-to)	419-427
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	204
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1994.2475
State	Published - Oct 15 1994

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "The cloning and expression of a human creatine transporter",

abstract = "A human creatine transporter (hCRT-BS2M) cDNA clone was isolated from a human brainstem/spinal cord using a PCR and phage plaque hybridization based technique. This clone included an open reading frame of 1,905 base pairs(bp) within a 2,283bp cDNA. Northern blot hybridization detected the expression of corresponding mRNAs most prominently in the skeletal muscle, heart and kidney. Peptide sequence analysis of the hCRT-BS2M protein product revealed 12 putative transmembrane domains. The predicted protein sequence further demonstrates that the hCRT-BS2M has highly conserved amino acid identity with the other members of the sodium dependent plasma membrane transporter family. Transient expression of the hCRT-BS2M in COS-7 cells demonstrates sodium dependent [14C]creatine uptake with a KM value of 14.9 ± 3.0 μM (n=5) that is attenuated by creatine and selective structural analogues of creatine.",

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AU - Sora, Ichiro

AU - Richman, Jeremy

AU - Santoro, Giovanna

AU - Wei, Hongbing

AU - Wang, Yu

AU - Vanderah, Todd

AU - Horvath, Robert

AU - Nguyen, Mike

AU - Waite, Sue

AU - Roeske, William R.

AU - Yamamura, Henry I.

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The cloning and expression of a human creatine transporter

Abstract

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