The capillary number effect on cell viability in Microfluidic Elasto-Filtration devices for viable circulating tumor cell isolation

Cong Zhao; Wenjuan Ma; Xingsu Yu; Huifang Su; Zhenfeng Zhang; Yitshak Zohar; Yi Kuen Lee

doi:10.1109/TRANSDUCERS.2017.7994092

The capillary number effect on cell viability in Microfluidic Elasto-Filtration devices for viable circulating tumor cell isolation

Cong Zhao, Wenjuan Ma, Xingsu Yu, Huifang Su, Zhenfeng Zhang, Yitshak Zohar, Yi Kuen Lee

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

This paper reports a systematic study of the Capillary number (Ca) effect on cell viability in Microfluidic Elasto-Filtration (MEF) devices for ex vivo circulating tumor cell (CTC) isolation. CTC viability decreases with increasing Ca because of the higher hydrodynamic shear stress. CTC viability also decreases with increasing cell diameter to filter-pore size ratio, d, due to the larger interaction forces between cells and filter pores. The cell viability dependence on Ca can be well fitted by two distinct functions for two ranges of d, larger and smaller than 2.5. Together with previously reported Ca effects on CTC capture efficiency and depletion of white blood cells, a complete design rule for MEF chips is proposed, yielding 92% CTC capture efficiency, 4-log WBC depletion, and 85% CTC viability at an optimized Ca.

Original language	English (US)
Title of host publication	TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	488-491
Number of pages	4
ISBN (Electronic)	9781538627310
DOIs	https://doi.org/10.1109/TRANSDUCERS.2017.7994092
State	Published - Jul 26 2017
Externally published	Yes
Event	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China Duration: Jun 18 2017 → Jun 22 2017

Publication series

Name	TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other	19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
Country/Territory	Taiwan, Province of China
City	Kaohsiung
Period	6/18/17 → 6/22/17

Keywords

Capillary number
Circulating tumor cells
Microfluidic Elasto-Filtration
Viability

ASJC Scopus subject areas

Chemical Health and Safety
Instrumentation
Electrical and Electronic Engineering

Access to Document

10.1109/TRANSDUCERS.2017.7994092

Cite this

Zhao, C., Ma, W., Yu, X., Su, H., Zhang, Z., Zohar, Y., & Lee, Y. K. (2017). The capillary number effect on cell viability in Microfluidic Elasto-Filtration devices for viable circulating tumor cell isolation. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 488-491). Article 7994092 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994092

The capillary number effect on cell viability in Microfluidic Elasto-Filtration devices for viable circulating tumor cell isolation. / Zhao, Cong; Ma, Wenjuan; Yu, Xingsu et al.
TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. p. 488-491 7994092 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Zhao, C, Ma, W, Yu, X, Su, H, Zhang, Z, Zohar, Y & Lee, YK 2017, The capillary number effect on cell viability in Microfluidic Elasto-Filtration devices for viable circulating tumor cell isolation. in TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems., 7994092, TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, Institute of Electrical and Electronics Engineers Inc., pp. 488-491, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, Kaohsiung, Taiwan, Province of China, 6/18/17. https://doi.org/10.1109/TRANSDUCERS.2017.7994092

Zhao C, Ma W, Yu X, Su H, Zhang Z, Zohar Y et al. The capillary number effect on cell viability in Microfluidic Elasto-Filtration devices for viable circulating tumor cell isolation. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc. 2017. p. 488-491. 7994092. (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). doi: 10.1109/TRANSDUCERS.2017.7994092

Zhao, Cong ; Ma, Wenjuan ; Yu, Xingsu et al. / The capillary number effect on cell viability in Microfluidic Elasto-Filtration devices for viable circulating tumor cell isolation. TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 488-491 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

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abstract = "This paper reports a systematic study of the Capillary number (Ca) effect on cell viability in Microfluidic Elasto-Filtration (MEF) devices for ex vivo circulating tumor cell (CTC) isolation. CTC viability decreases with increasing Ca because of the higher hydrodynamic shear stress. CTC viability also decreases with increasing cell diameter to filter-pore size ratio, d, due to the larger interaction forces between cells and filter pores. The cell viability dependence on Ca can be well fitted by two distinct functions for two ranges of d, larger and smaller than 2.5. Together with previously reported Ca effects on CTC capture efficiency and depletion of white blood cells, a complete design rule for MEF chips is proposed, yielding 92% CTC capture efficiency, 4-log WBC depletion, and 85% CTC viability at an optimized Ca.",

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AU - Zhao, Cong

AU - Ma, Wenjuan

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AU - Zhang, Zhenfeng

AU - Zohar, Yitshak

AU - Lee, Yi Kuen

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N2 - This paper reports a systematic study of the Capillary number (Ca) effect on cell viability in Microfluidic Elasto-Filtration (MEF) devices for ex vivo circulating tumor cell (CTC) isolation. CTC viability decreases with increasing Ca because of the higher hydrodynamic shear stress. CTC viability also decreases with increasing cell diameter to filter-pore size ratio, d, due to the larger interaction forces between cells and filter pores. The cell viability dependence on Ca can be well fitted by two distinct functions for two ranges of d, larger and smaller than 2.5. Together with previously reported Ca effects on CTC capture efficiency and depletion of white blood cells, a complete design rule for MEF chips is proposed, yielding 92% CTC capture efficiency, 4-log WBC depletion, and 85% CTC viability at an optimized Ca.

AB - This paper reports a systematic study of the Capillary number (Ca) effect on cell viability in Microfluidic Elasto-Filtration (MEF) devices for ex vivo circulating tumor cell (CTC) isolation. CTC viability decreases with increasing Ca because of the higher hydrodynamic shear stress. CTC viability also decreases with increasing cell diameter to filter-pore size ratio, d, due to the larger interaction forces between cells and filter pores. The cell viability dependence on Ca can be well fitted by two distinct functions for two ranges of d, larger and smaller than 2.5. Together with previously reported Ca effects on CTC capture efficiency and depletion of white blood cells, a complete design rule for MEF chips is proposed, yielding 92% CTC capture efficiency, 4-log WBC depletion, and 85% CTC viability at an optimized Ca.

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The capillary number effect on cell viability in Microfluidic Elasto-Filtration devices for viable circulating tumor cell isolation

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