TY - JOUR
T1 - The Bvg accessory factor (Baf) enhances pertussis toxin expression in Escherichia coli and is essential for Bordetella pertussis viability
AU - Wood, Gwendolyn E.
AU - Friedman, Richard L.
N1 - Funding Information:
The authors thank David DeShazer for construction of plasmid pDD100SN. We also thank Scott Stibitz for helpful discussions and James Moulder and Derek Wood for critical review of the manuscript. This work was supported by Grant AI37600 to R.L.F. from the National Institutes of Health.
PY - 2000/12/1
Y1 - 2000/12/1
N2 - Pertussis toxin expression in the Gram-negative respiratory pathogen, Bordetella pertussis, is regulated by the BvgAS two-component system. Previous studies suggested that an additional gene encoding a Bvg accessory factor (Baf) was required, along with BvgAS, for expression of a ptx-lacZ fusion in Escherichia coli grown in rich medium. However, other studies showed that BvgAS is sufficient for ptx-lacZ expression in minimal medium. Here we show that Baf acts with BvgAS to further increase ptx-lacZ expression in E. coli grown in minimal media and this is concomitant with a two-fold increase in BvgA protein levels. Gene replacement experiments show that baf is essential for viability of B. pertussis, suggesting that Baf affects the expression of other genes in addition to ptx. (C) 2000 Federation of European Microbiological Societies.
AB - Pertussis toxin expression in the Gram-negative respiratory pathogen, Bordetella pertussis, is regulated by the BvgAS two-component system. Previous studies suggested that an additional gene encoding a Bvg accessory factor (Baf) was required, along with BvgAS, for expression of a ptx-lacZ fusion in Escherichia coli grown in rich medium. However, other studies showed that BvgAS is sufficient for ptx-lacZ expression in minimal medium. Here we show that Baf acts with BvgAS to further increase ptx-lacZ expression in E. coli grown in minimal media and this is concomitant with a two-fold increase in BvgA protein levels. Gene replacement experiments show that baf is essential for viability of B. pertussis, suggesting that Baf affects the expression of other genes in addition to ptx. (C) 2000 Federation of European Microbiological Societies.
KW - Bordetella
KW - Bvg accessory factor
KW - BvgAS
KW - Pertussis toxin
KW - Two-component system
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U2 - 10.1016/S0378-1097(00)00448-1
DO - 10.1016/S0378-1097(00)00448-1
M3 - Article
C2 - 11094274
AN - SCOPUS:0034562175
SN - 0378-1097
VL - 193
SP - 25
EP - 30
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -