TY - JOUR
T1 - The anionic protease inhibitor BWI-1 from buckwheat seeds. Kinetic properties and possible biological role
AU - Dunaevsky, Yakov E.
AU - Gladysheva, Inna P.
AU - Pavlukova, Ekaterina B.
AU - Beliakova, Galina A.
AU - Gladyshev, Dmitry P.
AU - Papisova, Alla I.
AU - Larionova, Natalja I.
AU - Belozersky, Mikhail A.
PY - 1997
Y1 - 1997
N2 - Kinetic characteristics and effects on the growth of filamentous fungi of one of the main anionic protease inhibitors, BWI-1, isolated from buckwheat seeds, have been studied. The inhibition constants of bovine trypsin, chymotrypsin and cathepsin G from human granulocytes with BWI-1 were found to be 1.1, 67 and 200 nM, respectively. Analysis of the amino acid sequence of BWI-1 in the vicinity of the reactive site revealed its homology to the potato proteinase inhibitor I family. It is suggested that the inability of BWI-1 to bind elastase of human granulocytes is due to the basic nature of the amino acid residue (Arg) at the P1 position in its reactive site. It was demonstrated that BWI-1 was able to suppress the germination of the spores and the growth of the mycelium of two filamentous fungi.
AB - Kinetic characteristics and effects on the growth of filamentous fungi of one of the main anionic protease inhibitors, BWI-1, isolated from buckwheat seeds, have been studied. The inhibition constants of bovine trypsin, chymotrypsin and cathepsin G from human granulocytes with BWI-1 were found to be 1.1, 67 and 200 nM, respectively. Analysis of the amino acid sequence of BWI-1 in the vicinity of the reactive site revealed its homology to the potato proteinase inhibitor I family. It is suggested that the inability of BWI-1 to bind elastase of human granulocytes is due to the basic nature of the amino acid residue (Arg) at the P1 position in its reactive site. It was demonstrated that BWI-1 was able to suppress the germination of the spores and the growth of the mycelium of two filamentous fungi.
KW - Buckwheat
KW - Fagopyrum esculentum
KW - Filamentous fungi
KW - Proteinase
KW - Reactive site
KW - Seeds
KW - Trypsin inhibitor
UR - https://www.scopus.com/pages/publications/0031460521
UR - https://www.scopus.com/pages/publications/0031460521#tab=citedBy
U2 - 10.1034/j.1399-3054.1997.1010305.x
DO - 10.1034/j.1399-3054.1997.1010305.x
M3 - Article
AN - SCOPUS:0031460521
SN - 0031-9317
VL - 101
SP - 483
EP - 488
JO - Physiologia Plantarum
JF - Physiologia Plantarum
IS - 3
ER -