Texture analysis of speckle in optical coherence tomography images of tissue phantoms

Kirk W. Gossage, Cynthia M. Smith, Elizabeth M. Kanter, Lida P. Hariri, Alice L. Stone, Jeffrey J. Rodriguez, Stuart K. Williams, K. Barton Jennifer

Research output: Contribution to journalConference articlepeer-review

1 Scopus citations


Optical coherence tomography (OCT) is an imaging modality capable of acquiring cross-sectional images of tissue using back-reflected light. Conventional OCT images have a resolution of 10-15μm, and are thus best suited for visualizing tissue layers and structures. OCT images of collagen (with and without endothelial cells) have no resolvable features and may appear to simply show an exponential decrease in intensity with depth. However, examination of these images reveals that they display a characteristic repetitive structure due to speckle. The purpose of this study is to evaluate the application of statistical and spectral texture analysis techniques for differentiating living and non-living tissue phantoms containing various sizes and distributions of scatterers based on speckle content in OCT images. Statistically significant differences between texture parameters and excellent classification rates were obtained when comparing various endothelial cell concentrations ranging from 0 cells/ml to 25 million/ml. Statistically significant results and excellent classification rates were also obtained using various sizes of microspheres with concentrations ranging from 0 microspheres/ ml to 500 million microspheres/ml. This study has shown that texture analysis of OCT images may be capable of differentiating tissue phantoms containing various sizes and distributions of scatterers.

Original languageEnglish (US)
Pages (from-to)140-150
Number of pages11
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 2004
EventProgress in Biomedical Optics and Imaging - Adavnced Biomedical and Clinical Diagnostic Systems II - San Jose, CA, United States
Duration: Jan 25 2004Jan 26 2004


  • Collagen
  • Image Processing
  • Speckle
  • Texture Analysis
  • Tissue Phantoms

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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