Tetrahydroberberine blocks membrane K+ channels underlying its inhibition of intracellular message-mediated outward currents in acutely dissociated CA1 neurons from rat hippocampus

Jie Wu, Guo Zhang Jin

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

In the patch-clamp perforated whole-cell recording mode, tetrahydroberberine (THB), a novel dopamine (DA) receptor antagonist, inhibits not only DA-induced outward K+ currents, but also acetylcholine-, caffeine- or strychnine-induced outward current. However, THB does not affect either GABA- or glycine-induced Cl- currents, or non-NMDA receptor agonist- induced cation currents. As expected for a K+ channel blocker, THB evokes a downward current deflection accompanied by a decrease of conductance. It is concluded that the direct blockade of membrane K+ channels by THB underlies its inhibition of intracellular message-mediated outward currents.

Original languageEnglish (US)
Pages (from-to)214-218
Number of pages5
JournalBrain Research
Volume775
Issue number1-2
DOIs
StatePublished - Nov 14 1997
Externally publishedYes

Keywords

  • Dissociated neuron
  • Hippocampus
  • K channel
  • K current
  • Patch clamp
  • Tetrahydroberberine

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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