Tetraethylammonium transport by snake renal brush-border membrane vesicles

William H. Dantzler, Stephen H. Wright, Olga H. Brokl

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Transport of tetraethylammonium (TEA) by snake (Thamnophis spp.) renal brush-border membrane vesicles (BBMV) was studied. An outwardly directed proton gradient (pH 6.0 in, pH 7.5 out) stimulated uptake of TEA into BBMV and supported concentrative accumulation. Uptake of radioactively labeled TEA was also stimulated by outwardly directed gradients of unlabeled TEA and choline. The initial rate of TEA uptake was a saturable process that was adequately described by Michaelis-Menten kinetics. TEA uptake was not influenced by changes in the electrical potential difference across the membranes. Although uptake of TEA was stimulated by an outwardly directed Na+ gradient and inhibited by an inwardly directed Na+ gradient, these effects were probably secondary to the generation of proton gradients via a Na+/H+ exchanger demonstrated in these same BBMV. In agreement with previous studies with intact snake renal tubules, the present results indicate that TEA transport across the brush-border membrane involves electroneutral countertransport for protons or organic cations.

Original languageEnglish (US)
Pages (from-to)325-332
Number of pages8
JournalPflügers Archiv European Journal of Physiology
Issue number4
StatePublished - May 1991


  • Comparative renal physiology
  • Countertransport
  • Garter snake
  • Na/H exchange
  • Organic cation transport
  • Thamnophis spp

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)


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