TY - JOUR
T1 - Temporal progression of gene expression responses to salt shock in maize roots
AU - Wang, Hong
AU - Miyazaki, Saori
AU - Kawai, Kiyoshi
AU - Deyholos, Michael
AU - Galbraith, David W.
AU - Bohnert, Hans J.
PY - 2003/7
Y1 - 2003/7
N2 - Using a cDNA microarray containing 7943 ESTs, the behavior of the maize root transcriptome has been monitored in a time course for 72 h after imposition of salinity stress (150 mM NaCl). Under these conditions, root sodium amounts increased faster than in leaves, and root potassium decreased significantly. Although the overall free amino acid concentration was not affected, amino acid composition was changed with proline and asparagine increasing. Microarray analysis identified 916 ESTs representing genes whose steady-state RNA levels were significantly altered at various time points, corresponding to 11% of the ESTs printed. The response of the transcriptome to sub-lethal salt stress was rapid and transient, leading to a burst of changes at the three-hour time point. The salt-regulated ESTs represented 472 tentatively unique genes (TUGs), which, based on functional category analysis, are involved in a broad range of cellular and biochemical activities, prominent amongst which were transport and signal transduction pathways. Clustering of regulated transcripts based on the timing and duration of changes suggests a structured succession of induction and repression for salt responsive genes in multiple signal and response cascades. Within this framework, 16 signaling molecules, including six protein kinases, two protein phosphatases and eight transcription factors, were regulated with distinct expression patterns by high salinity.
AB - Using a cDNA microarray containing 7943 ESTs, the behavior of the maize root transcriptome has been monitored in a time course for 72 h after imposition of salinity stress (150 mM NaCl). Under these conditions, root sodium amounts increased faster than in leaves, and root potassium decreased significantly. Although the overall free amino acid concentration was not affected, amino acid composition was changed with proline and asparagine increasing. Microarray analysis identified 916 ESTs representing genes whose steady-state RNA levels were significantly altered at various time points, corresponding to 11% of the ESTs printed. The response of the transcriptome to sub-lethal salt stress was rapid and transient, leading to a burst of changes at the three-hour time point. The salt-regulated ESTs represented 472 tentatively unique genes (TUGs), which, based on functional category analysis, are involved in a broad range of cellular and biochemical activities, prominent amongst which were transport and signal transduction pathways. Clustering of regulated transcripts based on the timing and duration of changes suggests a structured succession of induction and repression for salt responsive genes in multiple signal and response cascades. Within this framework, 16 signaling molecules, including six protein kinases, two protein phosphatases and eight transcription factors, were regulated with distinct expression patterns by high salinity.
KW - Gene expression profile
KW - Microarray
KW - Salt stress
KW - Time course
KW - Zea mays
UR - http://www.scopus.com/inward/record.url?scp=0042194688&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0042194688&partnerID=8YFLogxK
U2 - 10.1023/A:1025029026375
DO - 10.1023/A:1025029026375
M3 - Article
C2 - 13677474
AN - SCOPUS:0042194688
VL - 52
SP - 873
EP - 891
JO - Plant Molecular Biology
JF - Plant Molecular Biology
SN - 0167-4412
IS - 4
ER -