Telomerase activity and differential expression of telomarase genes and c-myc in chicken cells in vitro

Susan E. Swanberg, William S. Payne, Henry D. Hunt, Jerry B. Dodgson, Mary E. Delany

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

This study examined telomerase activity and gene expression profiles for three genes in Galius gallus domesticus: telomerase reverse transcriptase (chTERT), telomerase RNA (chTR), and c-myc. Expression of these genes was studied in chicken embryonic stem (chES) cells, chicken embryo fibroblasts (CEFs), and DT40 cells using quantitative real-time polymerase chain reaction. Our results establish that, relative to transcription levels in telomerase-negative CEFs, chTERT and chTR are up-regulated in telomerase-positive chES cells. Transcription levels of chTERT, chTR, and c-myc are dramatically up-regulated in telomerase-positive DT40 cells, relative to CEFs and chES cells. These results are consistent with a model in which telomerase activity is up-regulated in proliferating embryonic stem cells requiring stable telomeres to endure multiple rounds of cell division; down-regulated in differentiated, lifespan-limited cells; and dramatically up-regulated in immortalized, transformed cells for which uncontrolled proliferation is correlated with c-myc dysregulaiion and telomerase activity.

Original languageEnglish (US)
Pages (from-to)14-21
Number of pages8
JournalDevelopmental Dynamics
Volume231
Issue number1
DOIs
StatePublished - Sep 2004
Externally publishedYes

Keywords

  • Chicken embryo fibroblasts
  • DT40
  • Embryonic stem cells
  • TERT
  • TR

ASJC Scopus subject areas

  • Developmental Biology

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