@inbook{0283e9f98ce34c2eba7853ba0d313395,
title = "Techniques to measure pilus retraction forces",
abstract = "The importance of physical forces in biology is becoming more appreciated. Neisseria gonorrhoeaehas become a paradigm for the study of physical forces in the bacterial world. Cycles of elongations and retractions of Type IV pili enables N. gonorrhoeaebacteria to exert forces on its environment, forces that play major roles in the life cycle of this pathogen. In order to better understand the role of these forces, there is a need to fully characterize them. Here, we present two different techniques, optical tweezers and Polyacrylamide MicroPillars (PoMPs), for measuring pilus retraction forces. Initially designed for N. gonorrhoeae, these assays can be readily modified to study other pilus-bearing bacteria including Neisseria meningitidis.",
keywords = "Neisseria, Optical tweezer, PoMPs, Retraction force, Type IV pilus",
author = "Nicolas Biais and Dustin Higashi and Magdalene So and Benoit Ladoux",
note = "Funding Information: N. B. and M. S. acknowledge the award of NIH grant AI079030. This work was also supported by the Agence Nationale de la Recherche(ANR)(ProgrammeBlanc2010SVSE5“MECANOCAD”) and the CNRS (Program Prise de Risques “Interface physique, biologie et chimie”). Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC 2012.",
year = "2012",
doi = "10.1007/978-1-61779-346-2_13",
language = "English (US)",
isbn = "9781617793455",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "197--216",
booktitle = "Neisseria meningitidis",
}