Techniques for following labeled cells in vivo: Use of X/Y FISH, techniques to optimize fluorescent detection, and beta-galactosidase detection

Michael Craig, Michael Schumacher, Yana Zavros

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The redistribution and trafficking patterns of cells to different anatomic sites throughout the body is important during cancer development and metastasis. Interest in the origin and fate of gastric cancer stem cells has recently arisen, as it may explain the underlying mechanism of cancer development. The ability to monitor the migration patterns of cancer stem cells is imperative to understanding the functional changes associated with the migration and proliferation of these cells. Here we detail a collection of techniques that include fluorescent in vivo imaging, X/Y FISH, and beta-galactosidase detection that are used for following labeled cells in vivo after adoptive transfer or transplant of donor cells for identifying the migration and engraftment of donor cells within the recipient.

Original languageEnglish (US)
Title of host publicationHelicobacter Species
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages227-238
Number of pages12
ISBN (Print)9781627030045
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume921
ISSN (Print)1064-3745

Keywords

  • Cancer stem cells
  • Fluorescence membrane labeling
  • Gastric cancer
  • Inflammation
  • Stomach

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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