TY - JOUR
T1 - TEA transport by snake renal tubules
T2 - Choline effects, countertransport, H+-TEA exchange
AU - Dantzler, W. H.
AU - Brokl, O. H.
PY - 1988
Y1 - 1988
N2 - Tetraethylammonium (TEA) transport by isolated perfused snake (Thamnophis spp.) proximal renal tubules was examined for effects of choline and evidence of countertransport of organic cations and H+-TEA exchange. Choline in perfusate significantly inhibited unidirectional lumen-to-bath flux of TEA and accumulation of labeled TEA by tubule cells, and choline in bath significantly inhibited unidirectional bath-to-lumen flux of TEA and accumulation of labeled TEA by tubule cells. These data indicate that choline inhibits transport of TEA into the cells across both the luminal and peritubular membranes and suggest that it may share the TEA transporter. To examine transport at single membranes in intact tubules, we examined the efflux of labeled TEA across the luminal membrane of tubules covered with oil and across the peritubular membrane of tubules with oil-filled lumens. Unlabeled TEA, choline, and low pH in the perfusate stimulated efflux of labeled TEA across the luminal membrane. These data suggest that TEA transport across the luminal membrane can involve countertransport of organic cations and H+-TEA exchange. Unlabeled TEA, choline, and, to a small extent, low pH in the bath stimulated the efflux of labeled TEA across the peritubular membrane. These data suggest that TEA transport across the peritubular membrane can involve countertransport of organic cations and, possibly,. H+-TEA exchange. The efflux data across both membranes further support the idea that choline may share the TEA transporter.
AB - Tetraethylammonium (TEA) transport by isolated perfused snake (Thamnophis spp.) proximal renal tubules was examined for effects of choline and evidence of countertransport of organic cations and H+-TEA exchange. Choline in perfusate significantly inhibited unidirectional lumen-to-bath flux of TEA and accumulation of labeled TEA by tubule cells, and choline in bath significantly inhibited unidirectional bath-to-lumen flux of TEA and accumulation of labeled TEA by tubule cells. These data indicate that choline inhibits transport of TEA into the cells across both the luminal and peritubular membranes and suggest that it may share the TEA transporter. To examine transport at single membranes in intact tubules, we examined the efflux of labeled TEA across the luminal membrane of tubules covered with oil and across the peritubular membrane of tubules with oil-filled lumens. Unlabeled TEA, choline, and low pH in the perfusate stimulated efflux of labeled TEA across the luminal membrane. These data suggest that TEA transport across the luminal membrane can involve countertransport of organic cations and H+-TEA exchange. Unlabeled TEA, choline, and, to a small extent, low pH in the bath stimulated the efflux of labeled TEA across the peritubular membrane. These data suggest that TEA transport across the peritubular membrane can involve countertransport of organic cations and, possibly,. H+-TEA exchange. The efflux data across both membranes further support the idea that choline may share the TEA transporter.
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U2 - 10.1152/ajprenal.1988.255.1.f167
DO - 10.1152/ajprenal.1988.255.1.f167
M3 - Article
C2 - 2839991
AN - SCOPUS:0023705982
SN - 0002-9513
VL - 255
SP - F167-F176
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
IS - 1 (24/1)
ER -