Abstract
Amyotrophic lateral sclerosis (ALS) is a genetically heterogeneous neurodegenerative disease in which 97% of patients exhibit cytoplasmic aggregates containing the RNA binding protein TDP-43. Using tagged ribosome affinity purifications in Drosophila models of TDP-43 proteinopathy, we identified TDP-43 dependent translational alterations in motor neurons impacting the spliceosome, pentose phosphate and oxidative phosphorylation pathways. A subset of the mRNAs with altered ribosome association are also enriched in TDP-43 complexes suggesting that they may be direct targets. Among these, dlp mRNA, which encodes the glypican Dally like protein (Dlp)/GPC6, a wingless (Wg/Wnt) signaling regulator is insolubilized both in flies and patient tissues with TDP-43 pathology. While Dlp/GPC6 forms puncta in the Drosophila neuropil and ALS spinal cords, it is reduced at the neuromuscular synapse in flies suggesting compartment specific effects of TDP-43 proteinopathy. These findings together with genetic interaction data show that Dlp/GPC6 is a novel, physiologically relevant target of TDP-43 proteinopathy.
Original language | English (US) |
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Article number | 52 |
Journal | Acta neuropathologica communications |
Volume | 9 |
Issue number | 1 |
DOIs | |
State | Published - Dec 2021 |
Keywords
- ALS
- Drosophila
- Glypican
- Motor neuron
- Neuromuscular junction
- TDP-43
- Translation
- Wnt signaling
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Clinical Neurology
- Cellular and Molecular Neuroscience
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Dive into the research topics of 'TDP-43 proteinopathy alters the ribosome association of multiple mRNAs including the glypican Dally-like protein (Dlp)/GPC6'. Together they form a unique fingerprint.Datasets
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Additional file 3 of TDP-43 proteinopathy alters the ribosome association of multiple mRNAs including the glypican Dally-like protein (Dlp)/GPC6
Lehmkuhl, E. M. (Creator), Loganathan, S. (Contributor), Alsop, E. (Creator), Blythe, A. D. (Creator), Kovalik, T. (Creator), Mortimore, N. P. (Creator), Barrameda, D. (Creator), Kueth, C. (Contributor), Eck, R. J. (Creator), Siddegowda, B. B. (Creator), Joardar, A. (Contributor), Ball, H. (Creator), Macias, M. E. (Creator), Bowser, R. (Creator), Van Keuren-Jensen, K. (Contributor), Zarnescu, D. C. (Creator) & Keuren-Jensen, K. V. (Creator), figshare, 2021
DOI: 10.6084/m9.figshare.14299509, https://springernature.figshare.com/articles/dataset/Additional_file_3_of_TDP-43_proteinopathy_alters_the_ribosome_association_of_multiple_mRNAs_including_the_glypican_Dally-like_protein_Dlp_GPC6/14299509
Dataset
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Additional file 1 of TDP-43 proteinopathy alters the ribosome association of multiple mRNAs including the glypican Dally-like protein (Dlp)/GPC6
Lehmkuhl, E. M. (Creator), Loganathan, S. (Contributor), Alsop, E. (Creator), Blythe, A. D. (Creator), Kovalik, T. (Creator), Mortimore, N. P. (Creator), Barrameda, D. (Creator), Kueth, C. (Contributor), Eck, R. J. (Creator), Siddegowda, B. B. (Creator), Joardar, A. (Contributor), Ball, H. (Creator), Macias, M. E. (Creator), Bowser, R. (Creator), Van Keuren-Jensen, K. (Contributor) & Zarnescu, D. C. (Creator), figshare, 2021
DOI: 10.6084/m9.figshare.14299503, https://springernature.figshare.com/articles/dataset/Additional_file_1_of_TDP-43_proteinopathy_alters_the_ribosome_association_of_multiple_mRNAs_including_the_glypican_Dally-like_protein_Dlp_GPC6/14299503
Dataset
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Additional file 2 of TDP-43 proteinopathy alters the ribosome association of multiple mRNAs including the glypican Dally-like protein (Dlp)/GPC6
Lehmkuhl, E. M. (Creator), Loganathan, S. (Contributor), Alsop, E. (Creator), Blythe, A. D. (Creator), Kovalik, T. (Creator), Mortimore, N. P. (Creator), Barrameda, D. (Creator), Kueth, C. (Contributor), Eck, R. J. (Creator), Siddegowda, B. B. (Creator), Joardar, A. (Contributor), Ball, H. (Creator), Macias, M. E. (Creator), Bowser, R. (Creator), Van Keuren-Jensen, K. (Contributor), Zarnescu, D. C. (Creator) & Keuren-Jensen, K. V. (Creator), figshare, 2021
DOI: 10.6084/m9.figshare.14299506, https://springernature.figshare.com/articles/dataset/Additional_file_2_of_TDP-43_proteinopathy_alters_the_ribosome_association_of_multiple_mRNAs_including_the_glypican_Dally-like_protein_Dlp_GPC6/14299506
Dataset