TCRβ enhancer activation occurs in some but not all cells with T cell lineage developmental potential

Hillary H. Norris; Lonnie P. Lybarger; Aaron J. Martin; Hanne Andersen; Deborah C. Chervenak; Robert Chervenak

doi:10.1016/S0008-8749(03)00113-8

TCRβ enhancer activation occurs in some but not all cells with T cell lineage developmental potential

Hillary H. Norris, Lonnie P. Lybarger, Aaron J. Martin, Hanne Andersen, Deborah C. Chervenak, Robert Chervenak

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Previous studies have shown that murine bone marrow contains a fraction of CD3^-/B220^-/Thy1^lo cells that have pre T cell activity following adoptive transfer and produce sterile transcripts of the T cell receptor β chain gene. The relationship between progenitors and TCRβ transcription has not been examined. Transgenic mice were generated that express green fluorescent protein under the control of the TCRβ enhancer (Eβ). Phenotypic analysis of the founders revealed faithful expression of GFP in populations that express TCRβ transcripts. Examination of the bone marrow showed two populations, CD3^-/B220^-/Thy1^- and CD3^-/B220^-/Thy1^lo, which were GFP⁺. Both populations were analyzed for their developmental potential following intrathymic transfer into recipient mice. Surprisingly, the GFP⁺/CD3^-/B220^-/Thy1^lo cells failed to reconstitute; however, the GFP⁺/CD3^-/B220^-/Thy1^- cells exhibited thymic repopulation. These data demonstrate that Eβ is active pre-thymically; however, pre-thymic transcription of the TCRβ chain gene is neither required for T cell development, nor is it limited to pre T cells.

Original language	English (US)
Pages (from-to)	164-174
Number of pages	11
Journal	Cellular Immunology
Volume	222
Issue number	2
DOIs	https://doi.org/10.1016/S0008-8749(03)00113-8
State	Published - Apr 2003
Externally published	Yes

Keywords

Hematopoiesis
Rodent
Stem cells
T cell receptors
T lymphocytes
Transgenic

ASJC Scopus subject areas

Immunology

Access to Document

10.1016/S0008-8749(03)00113-8

Cite this

@article{ef0107f7b1e14cf8a069a4e4e3a0d23d,

title = "TCRβ enhancer activation occurs in some but not all cells with T cell lineage developmental potential",

abstract = "Previous studies have shown that murine bone marrow contains a fraction of CD3-/B220-/Thy1lo cells that have pre T cell activity following adoptive transfer and produce sterile transcripts of the T cell receptor β chain gene. The relationship between progenitors and TCRβ transcription has not been examined. Transgenic mice were generated that express green fluorescent protein under the control of the TCRβ enhancer (Eβ). Phenotypic analysis of the founders revealed faithful expression of GFP in populations that express TCRβ transcripts. Examination of the bone marrow showed two populations, CD3-/B220-/Thy1- and CD3-/B220-/Thy1lo, which were GFP+. Both populations were analyzed for their developmental potential following intrathymic transfer into recipient mice. Surprisingly, the GFP+/CD3-/B220-/Thy1lo cells failed to reconstitute; however, the GFP+/CD3-/B220-/Thy1- cells exhibited thymic repopulation. These data demonstrate that Eβ is active pre-thymically; however, pre-thymic transcription of the TCRβ chain gene is neither required for T cell development, nor is it limited to pre T cells.",

keywords = "Hematopoiesis, Rodent, Stem cells, T cell receptors, T lymphocytes, Transgenic",

author = "Norris, {Hillary H.} and Lybarger, {Lonnie P.} and Martin, {Aaron J.} and Hanne Andersen and Chervenak, {Deborah C.} and Robert Chervenak",

note = "Funding Information: This work was in part supported by National Institutes of Health Grant RO1 AI31567. ",

year = "2003",

month = apr,

doi = "10.1016/S0008-8749(03)00113-8",

language = "English (US)",

volume = "222",

pages = "164--174",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - TCRβ enhancer activation occurs in some but not all cells with T cell lineage developmental potential

AU - Norris, Hillary H.

AU - Lybarger, Lonnie P.

AU - Martin, Aaron J.

AU - Andersen, Hanne

AU - Chervenak, Deborah C.

AU - Chervenak, Robert

N1 - Funding Information: This work was in part supported by National Institutes of Health Grant RO1 AI31567.

PY - 2003/4

Y1 - 2003/4

N2 - Previous studies have shown that murine bone marrow contains a fraction of CD3-/B220-/Thy1lo cells that have pre T cell activity following adoptive transfer and produce sterile transcripts of the T cell receptor β chain gene. The relationship between progenitors and TCRβ transcription has not been examined. Transgenic mice were generated that express green fluorescent protein under the control of the TCRβ enhancer (Eβ). Phenotypic analysis of the founders revealed faithful expression of GFP in populations that express TCRβ transcripts. Examination of the bone marrow showed two populations, CD3-/B220-/Thy1- and CD3-/B220-/Thy1lo, which were GFP+. Both populations were analyzed for their developmental potential following intrathymic transfer into recipient mice. Surprisingly, the GFP+/CD3-/B220-/Thy1lo cells failed to reconstitute; however, the GFP+/CD3-/B220-/Thy1- cells exhibited thymic repopulation. These data demonstrate that Eβ is active pre-thymically; however, pre-thymic transcription of the TCRβ chain gene is neither required for T cell development, nor is it limited to pre T cells.

AB - Previous studies have shown that murine bone marrow contains a fraction of CD3-/B220-/Thy1lo cells that have pre T cell activity following adoptive transfer and produce sterile transcripts of the T cell receptor β chain gene. The relationship between progenitors and TCRβ transcription has not been examined. Transgenic mice were generated that express green fluorescent protein under the control of the TCRβ enhancer (Eβ). Phenotypic analysis of the founders revealed faithful expression of GFP in populations that express TCRβ transcripts. Examination of the bone marrow showed two populations, CD3-/B220-/Thy1- and CD3-/B220-/Thy1lo, which were GFP+. Both populations were analyzed for their developmental potential following intrathymic transfer into recipient mice. Surprisingly, the GFP+/CD3-/B220-/Thy1lo cells failed to reconstitute; however, the GFP+/CD3-/B220-/Thy1- cells exhibited thymic repopulation. These data demonstrate that Eβ is active pre-thymically; however, pre-thymic transcription of the TCRβ chain gene is neither required for T cell development, nor is it limited to pre T cells.

KW - Hematopoiesis

KW - Rodent

KW - Stem cells

KW - T cell receptors

KW - T lymphocytes

KW - Transgenic

UR - http://www.scopus.com/inward/record.url?scp=0037783959&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037783959&partnerID=8YFLogxK

U2 - 10.1016/S0008-8749(03)00113-8

DO - 10.1016/S0008-8749(03)00113-8

M3 - Article

C2 - 12826086

AN - SCOPUS:0037783959

SN - 0008-8749

VL - 222

SP - 164

EP - 174

JO - Cellular Immunology

JF - Cellular Immunology

IS - 2

ER -

TCRβ enhancer activation occurs in some but not all cells with T cell lineage developmental potential

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this