Targeted next-generation sequencing of TP53 in oral tongue carcinoma from non-smokers

Daniel L. Faden; Sarah T. Arron; Chase M. Heaton; Joseph Derisi; Andrew P. South; Steven J. Wang

doi:10.1186/s40463-016-0160-4

Targeted next-generation sequencing of TP53 in oral tongue carcinoma from non-smokers

Daniel L. Faden, Sarah T. Arron, Chase M. Heaton, Joseph Derisi, Andrew P. South, Steven J. Wang

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Background: Little is known regarding the etiology and genomic underpinnings of Oral Tongue Squamous Cell Carcinoma (OTSCC) in patients who lack traditional risk factors, yet the incidence is increasing. In particular, the rate, and role, of TP53 mutations in this cohort has been heavily debated in the literature. Methods: Tumor DNA from forty-three non-smokers with OTSCC underwent next generation sequencing of TP53. Results: Sixty percent of samples contained a TP53 mutation. The G > T transversion rate was 5.7 %. TP53 status did not differ by age. Conclusions: OTSCC in non-smokers have TP53 mutation rates similar to other Head and Neck cancers yet these mutations do not appear related to carcinogen exposure based on the mutational spectrum and clinical history. The mechanisms driving tumorigenesis in this cohort, including mutations in TP53, remain elusive and further studies are needed.

Original language	English (US)
Article number	47
Journal	Journal of Otolaryngology - Head and Neck Surgery
Volume	45
Issue number	1
DOIs	https://doi.org/10.1186/s40463-016-0160-4
State	Published - Sep 17 2016
Externally published	Yes

Keywords

Oral tongue carcinoma
TP53

ASJC Scopus subject areas

Surgery
Otorhinolaryngology

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10.1186/s40463-016-0160-4

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@article{18b3aa19a5404baeb910269f18112784,

title = "Targeted next-generation sequencing of TP53 in oral tongue carcinoma from non-smokers",

abstract = "Background: Little is known regarding the etiology and genomic underpinnings of Oral Tongue Squamous Cell Carcinoma (OTSCC) in patients who lack traditional risk factors, yet the incidence is increasing. In particular, the rate, and role, of TP53 mutations in this cohort has been heavily debated in the literature. Methods: Tumor DNA from forty-three non-smokers with OTSCC underwent next generation sequencing of TP53. Results: Sixty percent of samples contained a TP53 mutation. The G > T transversion rate was 5.7 %. TP53 status did not differ by age. Conclusions: OTSCC in non-smokers have TP53 mutation rates similar to other Head and Neck cancers yet these mutations do not appear related to carcinogen exposure based on the mutational spectrum and clinical history. The mechanisms driving tumorigenesis in this cohort, including mutations in TP53, remain elusive and further studies are needed.",

keywords = "Oral tongue carcinoma, TP53",

author = "Faden, {Daniel L.} and Arron, {Sarah T.} and Heaton, {Chase M.} and Joseph Derisi and South, {Andrew P.} and Wang, {Steven J.}",

note = "Funding Information: Funding for this manuscript is from the personal research fund of SJW. There is no external grant support. Publisher Copyright: {\textcopyright} 2016 The Author(s).",

year = "2016",

month = sep,

day = "17",

doi = "10.1186/s40463-016-0160-4",

language = "English (US)",

volume = "45",

journal = "CANAD. J. OTOLARYNGOL.",

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publisher = "Decker Publishing",

number = "1",

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T1 - Targeted next-generation sequencing of TP53 in oral tongue carcinoma from non-smokers

AU - Faden, Daniel L.

AU - Arron, Sarah T.

AU - Heaton, Chase M.

AU - Derisi, Joseph

AU - South, Andrew P.

AU - Wang, Steven J.

PY - 2016/9/17

Y1 - 2016/9/17

N2 - Background: Little is known regarding the etiology and genomic underpinnings of Oral Tongue Squamous Cell Carcinoma (OTSCC) in patients who lack traditional risk factors, yet the incidence is increasing. In particular, the rate, and role, of TP53 mutations in this cohort has been heavily debated in the literature. Methods: Tumor DNA from forty-three non-smokers with OTSCC underwent next generation sequencing of TP53. Results: Sixty percent of samples contained a TP53 mutation. The G > T transversion rate was 5.7 %. TP53 status did not differ by age. Conclusions: OTSCC in non-smokers have TP53 mutation rates similar to other Head and Neck cancers yet these mutations do not appear related to carcinogen exposure based on the mutational spectrum and clinical history. The mechanisms driving tumorigenesis in this cohort, including mutations in TP53, remain elusive and further studies are needed.

AB - Background: Little is known regarding the etiology and genomic underpinnings of Oral Tongue Squamous Cell Carcinoma (OTSCC) in patients who lack traditional risk factors, yet the incidence is increasing. In particular, the rate, and role, of TP53 mutations in this cohort has been heavily debated in the literature. Methods: Tumor DNA from forty-three non-smokers with OTSCC underwent next generation sequencing of TP53. Results: Sixty percent of samples contained a TP53 mutation. The G > T transversion rate was 5.7 %. TP53 status did not differ by age. Conclusions: OTSCC in non-smokers have TP53 mutation rates similar to other Head and Neck cancers yet these mutations do not appear related to carcinogen exposure based on the mutational spectrum and clinical history. The mechanisms driving tumorigenesis in this cohort, including mutations in TP53, remain elusive and further studies are needed.

KW - Oral tongue carcinoma

KW - TP53

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DO - 10.1186/s40463-016-0160-4

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ER -

Targeted next-generation sequencing of TP53 in oral tongue carcinoma from non-smokers

Abstract

Keywords

ASJC Scopus subject areas

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