Taming of a poison: Biosynthesis of the NiFE-hydrogenase cyanide ligands

Stefanie Reissmann, Elisabeth Hochleitner, Haofan Wang, Athanasios Paschos, Friedrich Lottspeich, Richard S. Glass, August Böck

Research output: Contribution to journalArticlepeer-review

182 Scopus citations


NiFe-hydrogenases have an Ni-Fe site in which the iron has one CO and two CN groups as ligands. Synthesis of the CN ligands requires the activity of two hydrogenase maturation proteins: HypF and HypE. HypF is a carbamoyl-transferase that transfers the carbamoyl moiety of carbamoyladenylate to the COOH-terminal cysteine of HypE and thus forms an enzyme-thiocarbamate. HypE dehydrates the S-carbamoyl moiety in an adenosine triphosphate-dependent process to yield the enzyme thiocyanate. Chemical model reactions corroborate the feasibility of this unprecedented biosynthetic route and show that thiocyanates can donate CN to iron. This finding underscores a striking parallel between biochemistry and organometallic chemistry in the formation of an iron-cyano complex.

Original languageEnglish (US)
Pages (from-to)1067-1070
Number of pages4
Issue number5609
StatePublished - Feb 14 2003

ASJC Scopus subject areas

  • General


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