Tailoring the formation and stability of self-assembled structures from precisely engineered intrinsically disordered protein polymers: A comprehensive review

Fathima T. Doole, Christopher P. Camp, Minkyu Kim

Research output: Contribution to journalReview articlepeer-review

1 Scopus citations

Abstract

Intrinsically disordered proteins (IDPs) are a class of proteins that lack stable three-dimensional structures. Despite their natural tendency to be disordered, precise modulations of molecular parameters (e.g., sequence, length) through biomolecular engineering tools and control of environmental conditions tailor the formation of dynamic self-assembled structures. In addition to designing structures that respond to external stimuli for specific biotechnological applications (e.g., biosensors), other applications require stable structures (e.g., engineered tissues, drug delivery vehicles) that resist unintended changes and disassembly across various environmental conditions, such as different concentrations and temperatures. This review provides a comprehensive understanding of the design and engineering principles that govern the self-assembly of biosynthetic IDPs and their stability. Specifically, elastin-like polypeptides (ELPs) are highlighted as a prominent example of biosynthetically designed, thermoresponsive IDPs. Examples include ELPs that form various self-assembled structures by themselves as ELP homopolymers or diblock copolymers, ELPs combined with other IDPs in diblock copolymers, and ELP-based polymer hybrids containing functional (bio)molecules. It is anticipated that the efforts to enhance the stability of self-assembled structures through the precise engineering of IDP-based polymers have expanded the potential for diverse biotechnological applications in tissue engineering, drug delivery, diagnostic assays, and biomedicine.

Original languageEnglish (US)
Article number100158
JournalGiant
Volume14
DOIs
StatePublished - Jun 2023

Keywords

  • Intrinsically disordered protein
  • Protein polymer
  • Self-assembly
  • Stimulus responsiveness
  • Structural stability

ASJC Scopus subject areas

  • General Chemistry
  • Surfaces, Coatings and Films
  • Polymers and Plastics
  • Materials Chemistry

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