TY - JOUR
T1 - Synthesis, X-ray absorption spectroscopy and purification of the seleno-bis (S-glutathionyl) arsinium anion from selenide, arsenite and glutathione
AU - Gailer, Jürgen
AU - George, Graham N.
AU - Pickering, Ingrid J.
AU - Buttigieg, Gavin A.
AU - Denton, M. Bonner
AU - Glass, Richard S.
N1 - Funding Information:
Work at the University of Arizona was funded by Thermo Jarrel Ash Corporation (Franklyn Massachusetts, USA). Work at the Institute for Ecological Chemistry was supported by the Alexander von Humboldt Foundation. Work at SSRL was funded by the Department of Energy, Offices of Basic Energy Sciences and Biological and Environmental Research, and the National Institutes of Health, National Center for Research Resources, Biomedical Technology Program.
PY - 2002/5/1
Y1 - 2002/5/1
N2 - We report a new synthesis of the seleno-bis (S-glutathionyl) arsinium anion, [(GS)2AsSe]-. An aqueous solution of bis-glutathionylarsenous acid. (GS)2As-OH, prepared form stoichiometric glutathione and arsenite, was reacted in situ with a solution of sodium hydrogen selenide, prepared from elemental selenium and sodium borohydride. Analysis of the arsenic and selenium K-edge X-ray absorption spectra indicated virtually quantitative formation of [(GS)2AsSe]- with As Se and As-S distances of 2.31 and 2.25 Å, respectively, and the concentrated sample allowed a definitive X-ray spectroscopic characterization. Size-exclusion chromatography was used to separate [(GS)2AsSe]- from residual borate in the reaction mixture.
AB - We report a new synthesis of the seleno-bis (S-glutathionyl) arsinium anion, [(GS)2AsSe]-. An aqueous solution of bis-glutathionylarsenous acid. (GS)2As-OH, prepared form stoichiometric glutathione and arsenite, was reacted in situ with a solution of sodium hydrogen selenide, prepared from elemental selenium and sodium borohydride. Analysis of the arsenic and selenium K-edge X-ray absorption spectra indicated virtually quantitative formation of [(GS)2AsSe]- with As Se and As-S distances of 2.31 and 2.25 Å, respectively, and the concentrated sample allowed a definitive X-ray spectroscopic characterization. Size-exclusion chromatography was used to separate [(GS)2AsSe]- from residual borate in the reaction mixture.
KW - Arsinium anion
KW - S-glutathionyl
KW - Size-exclusion chromatography
KW - X-ray absorption spectroscopy
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U2 - 10.1016/S0022-328X(02)01192-0
DO - 10.1016/S0022-328X(02)01192-0
M3 - Article
AN - SCOPUS:0036569104
VL - 650
SP - 108
EP - 113
JO - Journal of Organometallic Chemistry
JF - Journal of Organometallic Chemistry
SN - 0022-328X
IS - 1-2
ER -