Synthesis, X-ray absorption spectroscopy and purification of the seleno-bis (S-glutathionyl) arsinium anion from selenide, arsenite and glutathione

Jürgen Gailer; Graham N. George; Ingrid J. Pickering; Gavin A. Buttigieg; M. Bonner Denton; Richard S Glass

doi:10.1016/S0022-328X(02)01192-0

Synthesis, X-ray absorption spectroscopy and purification of the seleno-bis (S-glutathionyl) arsinium anion from selenide, arsenite and glutathione

Jürgen Gailer, Graham N. George, Ingrid J. Pickering, Gavin A. Buttigieg, M. Bonner Denton, Richard S Glass

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

We report a new synthesis of the seleno-bis (S-glutathionyl) arsinium anion, [(GS)₂AsSe]^-. An aqueous solution of bis-glutathionylarsenous acid. (GS)₂As-OH, prepared form stoichiometric glutathione and arsenite, was reacted in situ with a solution of sodium hydrogen selenide, prepared from elemental selenium and sodium borohydride. Analysis of the arsenic and selenium K-edge X-ray absorption spectra indicated virtually quantitative formation of [(GS)₂AsSe]^- with As Se and As-S distances of 2.31 and 2.25 Å, respectively, and the concentrated sample allowed a definitive X-ray spectroscopic characterization. Size-exclusion chromatography was used to separate [(GS)₂AsSe]- from residual borate in the reaction mixture.

Original language	English (US)
Pages (from-to)	108-113
Number of pages	6
Journal	Journal of Organometallic Chemistry
Volume	650
Issue number	1-2
DOIs	https://doi.org/10.1016/S0022-328X(02)01192-0
State	Published - May 1 2002
Externally published	Yes

Keywords

Arsinium anion
S-glutathionyl
Size-exclusion chromatography
X-ray absorption spectroscopy

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
Materials Chemistry

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10.1016/S0022-328X(02)01192-0

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title = "Synthesis, X-ray absorption spectroscopy and purification of the seleno-bis (S-glutathionyl) arsinium anion from selenide, arsenite and glutathione",

abstract = "We report a new synthesis of the seleno-bis (S-glutathionyl) arsinium anion, [(GS)2AsSe]-. An aqueous solution of bis-glutathionylarsenous acid. (GS)2As-OH, prepared form stoichiometric glutathione and arsenite, was reacted in situ with a solution of sodium hydrogen selenide, prepared from elemental selenium and sodium borohydride. Analysis of the arsenic and selenium K-edge X-ray absorption spectra indicated virtually quantitative formation of [(GS)2AsSe]- with As Se and As-S distances of 2.31 and 2.25 {\AA}, respectively, and the concentrated sample allowed a definitive X-ray spectroscopic characterization. Size-exclusion chromatography was used to separate [(GS)2AsSe]- from residual borate in the reaction mixture.",

keywords = "Arsinium anion, S-glutathionyl, Size-exclusion chromatography, X-ray absorption spectroscopy",

author = "J{\"u}rgen Gailer and George, {Graham N.} and Pickering, {Ingrid J.} and Buttigieg, {Gavin A.} and Denton, {M. Bonner} and Glass, {Richard S}",

note = "Funding Information: Work at the University of Arizona was funded by Thermo Jarrel Ash Corporation (Franklyn Massachusetts, USA). Work at the Institute for Ecological Chemistry was supported by the Alexander von Humboldt Foundation. Work at SSRL was funded by the Department of Energy, Offices of Basic Energy Sciences and Biological and Environmental Research, and the National Institutes of Health, National Center for Research Resources, Biomedical Technology Program.",

year = "2002",

month = may,

day = "1",

doi = "10.1016/S0022-328X(02)01192-0",

language = "English (US)",

volume = "650",

pages = "108--113",

journal = "Journal of Organometallic Chemistry",

issn = "0022-328X",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - Synthesis, X-ray absorption spectroscopy and purification of the seleno-bis (S-glutathionyl) arsinium anion from selenide, arsenite and glutathione

AU - Gailer, Jürgen

AU - George, Graham N.

AU - Pickering, Ingrid J.

AU - Buttigieg, Gavin A.

AU - Denton, M. Bonner

AU - Glass, Richard S

N1 - Funding Information: Work at the University of Arizona was funded by Thermo Jarrel Ash Corporation (Franklyn Massachusetts, USA). Work at the Institute for Ecological Chemistry was supported by the Alexander von Humboldt Foundation. Work at SSRL was funded by the Department of Energy, Offices of Basic Energy Sciences and Biological and Environmental Research, and the National Institutes of Health, National Center for Research Resources, Biomedical Technology Program.

PY - 2002/5/1

Y1 - 2002/5/1

N2 - We report a new synthesis of the seleno-bis (S-glutathionyl) arsinium anion, [(GS)2AsSe]-. An aqueous solution of bis-glutathionylarsenous acid. (GS)2As-OH, prepared form stoichiometric glutathione and arsenite, was reacted in situ with a solution of sodium hydrogen selenide, prepared from elemental selenium and sodium borohydride. Analysis of the arsenic and selenium K-edge X-ray absorption spectra indicated virtually quantitative formation of [(GS)2AsSe]- with As Se and As-S distances of 2.31 and 2.25 Å, respectively, and the concentrated sample allowed a definitive X-ray spectroscopic characterization. Size-exclusion chromatography was used to separate [(GS)2AsSe]- from residual borate in the reaction mixture.

AB - We report a new synthesis of the seleno-bis (S-glutathionyl) arsinium anion, [(GS)2AsSe]-. An aqueous solution of bis-glutathionylarsenous acid. (GS)2As-OH, prepared form stoichiometric glutathione and arsenite, was reacted in situ with a solution of sodium hydrogen selenide, prepared from elemental selenium and sodium borohydride. Analysis of the arsenic and selenium K-edge X-ray absorption spectra indicated virtually quantitative formation of [(GS)2AsSe]- with As Se and As-S distances of 2.31 and 2.25 Å, respectively, and the concentrated sample allowed a definitive X-ray spectroscopic characterization. Size-exclusion chromatography was used to separate [(GS)2AsSe]- from residual borate in the reaction mixture.

KW - Arsinium anion

KW - S-glutathionyl

KW - Size-exclusion chromatography

KW - X-ray absorption spectroscopy

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U2 - 10.1016/S0022-328X(02)01192-0

DO - 10.1016/S0022-328X(02)01192-0

M3 - Article

AN - SCOPUS:0036569104

SN - 0022-328X

VL - 650

SP - 108

EP - 113

JO - Journal of Organometallic Chemistry

JF - Journal of Organometallic Chemistry

IS - 1-2

ER -

Synthesis, X-ray absorption spectroscopy and purification of the seleno-bis (S-glutathionyl) arsinium anion from selenide, arsenite and glutathione

Abstract

Keywords

ASJC Scopus subject areas

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