Synthesis of procaspases-3 and -7 during apoptosis in prostate cancer cells

Cai Bowen, H. James Voeller, Kristine Kikly, Edward P. Gelmann

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Cells differ in the time required to execute cell death after receipt of a death signal. One reason may be the requirement for de novo synthesis of components of the death pathway. TSU-Pr1 prostate cancer cells treated with okadaic acid demonstrated activation of caspase-3, PARP cleavage, and nuclear fragmentation by 24 h and apoptosis by 72 h. Levels of procaspase-3 and procaspase-7, the precursor molecules of two effector caspases, were not depleted during apoptosis. Levels of procaspase-3 and -7 mRNA increased steadily in ISU-Pr1 cells up to 72 h after exposure to okadaic acid. Nuclear run-off experiments showed that the increase in mRNA was not due to transcriptional activation of caspase-3 and -7 mRNA. Antisense caspase-3 and caspase-7 oligodeoxynucleotides caused a depletion of procaspases-3 and -7 and a delay in apoptosis of TSU-Pr1 cells. Caspase antisense oligodeoxynucleotides inhibited apoptosis to a similar extent as peptide inhibitors of cysteine proteases. Synthesis of procaspases-3 and -7 was necessary to sustain programmed cell death in TSU-Pr1 prostate cancer cells.

Original languageEnglish (US)
Pages (from-to)394-401
Number of pages8
JournalCell Death and Differentiation
Volume6
Issue number5
DOIs
StatePublished - May 1999
Externally publishedYes

Keywords

  • Antisense
  • Apoptosis
  • Caspase
  • Prostate cancer

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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