TY - JOUR
T1 - Synthesis and biological actions of melanin concentrating hormone
AU - Wilkes, Brian C.
AU - Hruby, Victor J.
AU - Sherbrooke, Wade C.
AU - Castrucci, Ana M.de L.
AU - Hadley, M. E.
N1 - Funding Information:
Supported in part by U.S. Public Health Service grant AM17420 and by National Science Foundation grants PCM-8112200 and PCM-8110708. Dr. A.M. Castrucci is a fellow of the Conselho Nacional de Desenvolvimento Tecnologlco / . e Cientifico 1 of Brasil, grant 200 430/82.
PY - 1984/7/31
Y1 - 1984/7/31
N2 - A melanin (melanosome) concentrating hormone, MCH, was synthesized and the methodology for its synthesis is detailed. This heptadecapeptide, H-Asp-Thr-Met-Arg-Cys-Met-Val-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Glu-Val-OH, stimulated melanosome concentration (centripetal aggregation) within melanophores of all species of teleost fishes studied. Melanosome aggregation in response to MCH was not blocked by Dibenamine as was the response to norepinephrine (NE), demonstrating that melanosome aggregating responses to MCH and NE are mediated through separate receptors. Melanosome aggregation in response to MCH was reversed by an equimolar concentration of α-melanocyte stimulating hormone (α-MSH). In contrast, MCH stimulated melanosome dispersion (centrifugal movement) within melanophores of a frog (Rana pipiens) and a lizard (Anolis carolinensis). Therefore, MCH exhibits both melanosome concentrating and dispersing actions depending upon the species studied.
AB - A melanin (melanosome) concentrating hormone, MCH, was synthesized and the methodology for its synthesis is detailed. This heptadecapeptide, H-Asp-Thr-Met-Arg-Cys-Met-Val-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp-Glu-Val-OH, stimulated melanosome concentration (centripetal aggregation) within melanophores of all species of teleost fishes studied. Melanosome aggregation in response to MCH was not blocked by Dibenamine as was the response to norepinephrine (NE), demonstrating that melanosome aggregating responses to MCH and NE are mediated through separate receptors. Melanosome aggregation in response to MCH was reversed by an equimolar concentration of α-melanocyte stimulating hormone (α-MSH). In contrast, MCH stimulated melanosome dispersion (centrifugal movement) within melanophores of a frog (Rana pipiens) and a lizard (Anolis carolinensis). Therefore, MCH exhibits both melanosome concentrating and dispersing actions depending upon the species studied.
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U2 - 10.1016/S0006-291X(84)80077-7
DO - 10.1016/S0006-291X(84)80077-7
M3 - Article
C2 - 6466330
AN - SCOPUS:0021132363
SN - 0006-291X
VL - 122
SP - 613
EP - 619
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -