TY - JOUR
T1 - Synthesis and antitumor activity of heterocycles related to carbendazim
AU - Remers, William A.
AU - Iyengar, Bhashyam S.
AU - Dorr, Robert T.
AU - Wisner, Lee
AU - Bates, Robert B.
N1 - Publisher Copyright:
© 2014 HeteroCorporation.
PY - 2015/1
Y1 - 2015/1
N2 - Three types of compounds were synthesized from carbendazim (1), a benzimidazole derivative (Scheme 1). They included a group of esters at N1 prepared by treating carbendazim with isocyanates bearing ester groups (2a-2c), carboxyalkyl-1,2,3,4-tetrahydro-s-triazino[1,2-a]benzimidazole-2,4-dione esters (3a and 3d) formed by cyclization of two of these esters, and related hydrophilic amides 3b and 3c derived from 3a. The antitumor potencies of the N1 esters were in the range of 7 to 40 mM, which compares favorably with carbendazim, but their water solubilities were low. The s-triazine derivatives showed activity against pancreatic tumor cells, and one of them (3b) was active in mice, but they were not effective against other tumor types. Treatment of carbendazim with 3-bromopropionyl chloride produced 1-methoxycarbonyl- 4-oxo-1,2,3,4-tetrahydropyrimido[1,2-a]benzimidazole (4), which gave 1-(3-aminopropionyl)benzimidazole 2-methylcarbamates, substituted on the amino nitrogen (5a, 5b, and 5d), when treated with amines. These products showed some antitumor activity in cell cultures, and an ethoxy derivative (5c), obtained by treating 1-methoxycarbonyl-4-oxo-1,2,3,4-tetrahydropyrimido[1,2-a]benzimidazole with sodium ethoxide, was active in the 67-150 mM range. Some of the new compounds had good water solubility. Carbendazim kills tumor cells by inhibiting tubulin; however, s-triazine 3b, which differs from it in size and functional groups, does not act by this mechanism.
AB - Three types of compounds were synthesized from carbendazim (1), a benzimidazole derivative (Scheme 1). They included a group of esters at N1 prepared by treating carbendazim with isocyanates bearing ester groups (2a-2c), carboxyalkyl-1,2,3,4-tetrahydro-s-triazino[1,2-a]benzimidazole-2,4-dione esters (3a and 3d) formed by cyclization of two of these esters, and related hydrophilic amides 3b and 3c derived from 3a. The antitumor potencies of the N1 esters were in the range of 7 to 40 mM, which compares favorably with carbendazim, but their water solubilities were low. The s-triazine derivatives showed activity against pancreatic tumor cells, and one of them (3b) was active in mice, but they were not effective against other tumor types. Treatment of carbendazim with 3-bromopropionyl chloride produced 1-methoxycarbonyl- 4-oxo-1,2,3,4-tetrahydropyrimido[1,2-a]benzimidazole (4), which gave 1-(3-aminopropionyl)benzimidazole 2-methylcarbamates, substituted on the amino nitrogen (5a, 5b, and 5d), when treated with amines. These products showed some antitumor activity in cell cultures, and an ethoxy derivative (5c), obtained by treating 1-methoxycarbonyl-4-oxo-1,2,3,4-tetrahydropyrimido[1,2-a]benzimidazole with sodium ethoxide, was active in the 67-150 mM range. Some of the new compounds had good water solubility. Carbendazim kills tumor cells by inhibiting tubulin; however, s-triazine 3b, which differs from it in size and functional groups, does not act by this mechanism.
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U2 - 10.1002/jhet.1976
DO - 10.1002/jhet.1976
M3 - Article
AN - SCOPUS:84921525004
SN - 0022-152X
VL - 52
SP - 136
EP - 141
JO - Journal of Heterocyclic Chemistry
JF - Journal of Heterocyclic Chemistry
IS - 1
ER -