TY - JOUR
T1 - Synergistic interaction of clavata1, clavata2, and receptor-like protein kinase 2 in cyst nematode parasitism of arabidopsis
AU - Replogle, Amy
AU - Wang, Jianying
AU - Paolillo, Vitoria
AU - Smeda, John
AU - Kinoshita, Atsuko
AU - Durbak, Amanda
AU - Tax, Frans E.
AU - Wang, Xiaohong
AU - Sawa, Shinichiro
AU - Mitchum, Melissa G.
PY - 2013/1
Y1 - 2013/1
N2 - Plant-parasitic cyst nematodes secrete CLAVATA3 (CLV3)/ ENDOSPERM SURROUNDING REGION (CLE)-like effector proteins. These proteins act as ligand mimics of plant CLE peptides and are required for successful nematode infection. Previously, we showed that the CLV2/ CORYNE (CRN) heterodimer receptor complex is required for nematode CLE signaling. However, there was only a partial reduction in nematode infection when this signaling was disrupted, indicating that there might be additional nematode CLE receptors. In this study, we demonstrate that CLV1 and RECEPTOR-LIKE PROTEIN KINASE 2/TOADSTOOL2 (RPK2), two additional receptors that can transmit the CLV3 signal independent of CLV2/CRN for shoot apical meristem maintenance, also play a role in nematode CLE perception. Localization studies showed that both receptors are expressed in nematode-induced syncytia. Infection assays with clv1 and rpk2 single mutants revealed a decrease in both nematode infection and syncytium size. Significantly, further reduction in nematode infection was observed when rpk2 was combined with clv1 and clv2 mutants. Taken together, our results indicate that parallel signaling pathways involving CLV1, CLV2, and RPK2 are important for nematode parasitism.
AB - Plant-parasitic cyst nematodes secrete CLAVATA3 (CLV3)/ ENDOSPERM SURROUNDING REGION (CLE)-like effector proteins. These proteins act as ligand mimics of plant CLE peptides and are required for successful nematode infection. Previously, we showed that the CLV2/ CORYNE (CRN) heterodimer receptor complex is required for nematode CLE signaling. However, there was only a partial reduction in nematode infection when this signaling was disrupted, indicating that there might be additional nematode CLE receptors. In this study, we demonstrate that CLV1 and RECEPTOR-LIKE PROTEIN KINASE 2/TOADSTOOL2 (RPK2), two additional receptors that can transmit the CLV3 signal independent of CLV2/CRN for shoot apical meristem maintenance, also play a role in nematode CLE perception. Localization studies showed that both receptors are expressed in nematode-induced syncytia. Infection assays with clv1 and rpk2 single mutants revealed a decrease in both nematode infection and syncytium size. Significantly, further reduction in nematode infection was observed when rpk2 was combined with clv1 and clv2 mutants. Taken together, our results indicate that parallel signaling pathways involving CLV1, CLV2, and RPK2 are important for nematode parasitism.
UR - http://www.scopus.com/inward/record.url?scp=84870975469&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870975469&partnerID=8YFLogxK
U2 - 10.1094/MPMI-05-12-0118-FI
DO - 10.1094/MPMI-05-12-0118-FI
M3 - Article
C2 - 22835273
AN - SCOPUS:84870975469
SN - 0894-0282
VL - 26
SP - 87
EP - 96
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
IS - 1
ER -