Synaptic resolution two-photon stimulation and imaging of neural activity with Bessel beam light-sheet microscopy

Dongli Xu; Jun B. Ding; Leilei Peng

doi:10.1364/BRAIN.2023.BW4B.3

Synaptic resolution two-photon stimulation and imaging of neural activity with Bessel beam light-sheet microscopy

Dongli Xu, Jun B. Ding, Leilei Peng

Research output: Contribution to conference › Paper › peer-review

Abstract

We report combining two-photon Bessel beam light-sheet microscopy with two-photon uncaging to perform all-optical neural stimulation and imaging at synaptic resolution. Imaging results from mouse brain slices under 2P uncaging of glutamate will be presented.

Original language	English (US)
DOIs	https://doi.org/10.1364/BRAIN.2023.BW4B.3
State	Published - 2023
Event	Biophotonics Congress: Optics in the Life Sciences 2023, OMA, NTM, BODA, OMP, BRAIN 2023 - Part of Optica Biophotonics Congress: Optics in the Life Sciences - Vancouver, Canada Duration: Apr 24 2023 → Apr 27 2023

Conference

Conference	Biophotonics Congress: Optics in the Life Sciences 2023, OMA, NTM, BODA, OMP, BRAIN 2023 - Part of Optica Biophotonics Congress: Optics in the Life Sciences
Country/Territory	Canada
City	Vancouver
Period	4/24/23 → 4/27/23

ASJC Scopus subject areas

General Computer Science
Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Space and Planetary Science
Control and Systems Engineering
Electrical and Electronic Engineering
Instrumentation

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10.1364/BRAIN.2023.BW4B.3

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Xu, D., Ding, J. B., & Peng, L. (2023). Synaptic resolution two-photon stimulation and imaging of neural activity with Bessel beam light-sheet microscopy. Paper presented at Biophotonics Congress: Optics in the Life Sciences 2023, OMA, NTM, BODA, OMP, BRAIN 2023 - Part of Optica Biophotonics Congress: Optics in the Life Sciences, Vancouver, Canada. https://doi.org/10.1364/BRAIN.2023.BW4B.3

Synaptic resolution two-photon stimulation and imaging of neural activity with Bessel beam light-sheet microscopy. / Xu, Dongli; Ding, Jun B.; Peng, Leilei.
2023. Paper presented at Biophotonics Congress: Optics in the Life Sciences 2023, OMA, NTM, BODA, OMP, BRAIN 2023 - Part of Optica Biophotonics Congress: Optics in the Life Sciences, Vancouver, Canada.

Research output: Contribution to conference › Paper › peer-review

Xu, D, Ding, JB & Peng, L 2023, 'Synaptic resolution two-photon stimulation and imaging of neural activity with Bessel beam light-sheet microscopy', Paper presented at Biophotonics Congress: Optics in the Life Sciences 2023, OMA, NTM, BODA, OMP, BRAIN 2023 - Part of Optica Biophotonics Congress: Optics in the Life Sciences, Vancouver, Canada, 4/24/23 - 4/27/23. https://doi.org/10.1364/BRAIN.2023.BW4B.3

Xu D, Ding JB, Peng L. Synaptic resolution two-photon stimulation and imaging of neural activity with Bessel beam light-sheet microscopy. 2023. Paper presented at Biophotonics Congress: Optics in the Life Sciences 2023, OMA, NTM, BODA, OMP, BRAIN 2023 - Part of Optica Biophotonics Congress: Optics in the Life Sciences, Vancouver, Canada. doi: 10.1364/BRAIN.2023.BW4B.3

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abstract = "We report combining two-photon Bessel beam light-sheet microscopy with two-photon uncaging to perform all-optical neural stimulation and imaging at synaptic resolution. Imaging results from mouse brain slices under 2P uncaging of glutamate will be presented.",

author = "Dongli Xu and Ding, {Jun B.} and Leilei Peng",

note = "Publisher Copyright: {\textcopyright} 2023 The Author(s), {\textcopyright} Optica Publishing Group 2023.; Biophotonics Congress: Optics in the Life Sciences 2023, OMA, NTM, BODA, OMP, BRAIN 2023 - Part of Optica Biophotonics Congress: Optics in the Life Sciences ; Conference date: 24-04-2023 Through 27-04-2023",

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Synaptic resolution two-photon stimulation and imaging of neural activity with Bessel beam light-sheet microscopy

Abstract

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ASJC Scopus subject areas

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