Surface modification of electrospun scaffolds for endothelialization of tissue-engineered vascular grafts using human cord blood-derived endothelial cells

Diana Catalina Ardila, Jr Jiun Liou, David Maestas, Marvin J. Slepian, Michael Badowski, William R. Wagner, David Harris, Jonathan P.Vande Geest

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Tissue engineering has gained attention as an alternative approach for developing small diameter tissue-engineered vascular grafts intended for bypass surgery, as an option to treat coronary heart disease. To promote the formation of a healthy endothelial cell monolayer in the lumen of the graft, polycaprolactone/gelatin/fibrinogen scaffolds were developed, and the surface was modified using thermoforming and coating with collagen IV and fibronectin. Human cord blood-derived endothelial cells (hCB-ECs) were seeded onto the scaffolds and the important characteristics of a healthy endothelial cell layer were evaluated under static conditions using human umbilical vein endothelial cells as a control. We found that polycaprolactone/gelatin/fibrinogen scaffolds that were thermoformed and coated are the most suitable for endothelial cell growth. hCB-ECs can proliferate, produce endothelial nitric oxide synthase, respond to interleukin 1 beta, and reduce platelet deposition.

Original languageEnglish (US)
Article number185
JournalJournal of Clinical Medicine
Volume8
Issue number2
DOIs
StatePublished - 2019

Keywords

  • Endothelialization
  • Human cord blood-derived endothelial cells
  • Umbilical cord blood
  • Vascular graft
  • Vascular tissue engineering

ASJC Scopus subject areas

  • Medicine(all)

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