Abstract
Tissue engineering has gained attention as an alternative approach for developing small diameter tissue-engineered vascular grafts intended for bypass surgery, as an option to treat coronary heart disease. To promote the formation of a healthy endothelial cell monolayer in the lumen of the graft, polycaprolactone/gelatin/fibrinogen scaffolds were developed, and the surface was modified using thermoforming and coating with collagen IV and fibronectin. Human cord blood-derived endothelial cells (hCB-ECs) were seeded onto the scaffolds and the important characteristics of a healthy endothelial cell layer were evaluated under static conditions using human umbilical vein endothelial cells as a control. We found that polycaprolactone/gelatin/fibrinogen scaffolds that were thermoformed and coated are the most suitable for endothelial cell growth. hCB-ECs can proliferate, produce endothelial nitric oxide synthase, respond to interleukin 1 beta, and reduce platelet deposition.
Original language | English (US) |
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Article number | 185 |
Journal | Journal of Clinical Medicine |
Volume | 8 |
Issue number | 2 |
DOIs | |
State | Published - 2019 |
Keywords
- Endothelialization
- Human cord blood-derived endothelial cells
- Umbilical cord blood
- Vascular graft
- Vascular tissue engineering
ASJC Scopus subject areas
- General Medicine