Surface analysis of early and late MOPC-315-EL tumor cells

David Harris; Jennifer Bailey; Jon C. Lewis; William H. Grover; Marguerite Panetti; Donald L. Evans; Mary J. Ruebush; Leslie K. Paulus; Donald Burgess; Arthur H. Hale

doi:10.1016/0008-8749(81)90463-9

Surface analysis of early and late MOPC-315-EL tumor cells

David Harris, Jennifer Bailey, Jon C. Lewis, William H. Grover, Marguerite Panetti, Donald L. Evans, Mary J. Ruebush, Leslie K. Paulus, Donald Burgess, Arthur H. Hale

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The surface properties of a tumor line which undergoes a cyclic change in reactivity with cytotoxic T lymphocytes (CTLs) have been analyzed. The findings indicate that MOPC-315-EL when transplanted into the peritoneum of a BALB/c mouse for 4-5 days (early MOPC315-EL) becomes sensitive to lysis by anti-H-2^d CTL. After 9 to 10 days of growth in the peritoneal cavity these tumor cells (late MOPC-315-EL) become unreactive to CTLs. The amount of H-2 antigens on the surface of the cells is the same on the early and late tumor cells. By scanning electron microscopy (SEM) we have shown that the early population is a mixture of two cell types which are characterized by surface villi or surface blebs; the late cells are essentially one population which are characterized by surface villi. The net charge on the surface of the cell was evaluated by two methods: (i) rate of cell migration across an electric potential and (ii) binding by cationic ferritin to the glycocalyx. Both techniques indicated that the early cells have a higher net negative charge. The implication of this work is that surface charge may be involved in T cell-target cell interactions.

Original language	English (US)
Pages (from-to)	119-130
Number of pages	12
Journal	Cellular Immunology
Volume	64
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(81)90463-9
State	Published - Oct 1981
Externally published	Yes

ASJC Scopus subject areas

Immunology

Access to Document

10.1016/0008-8749(81)90463-9

Cite this

@article{5e6ab42a8e814e55b9e87190c757d177,

title = "Surface analysis of early and late MOPC-315-EL tumor cells",

abstract = "The surface properties of a tumor line which undergoes a cyclic change in reactivity with cytotoxic T lymphocytes (CTLs) have been analyzed. The findings indicate that MOPC-315-EL when transplanted into the peritoneum of a BALB/c mouse for 4-5 days (early MOPC315-EL) becomes sensitive to lysis by anti-H-2d CTL. After 9 to 10 days of growth in the peritoneal cavity these tumor cells (late MOPC-315-EL) become unreactive to CTLs. The amount of H-2 antigens on the surface of the cells is the same on the early and late tumor cells. By scanning electron microscopy (SEM) we have shown that the early population is a mixture of two cell types which are characterized by surface villi or surface blebs; the late cells are essentially one population which are characterized by surface villi. The net charge on the surface of the cell was evaluated by two methods: (i) rate of cell migration across an electric potential and (ii) binding by cationic ferritin to the glycocalyx. Both techniques indicated that the early cells have a higher net negative charge. The implication of this work is that surface charge may be involved in T cell-target cell interactions.",

author = "David Harris and Jennifer Bailey and Lewis, {Jon C.} and Grover, {William H.} and Marguerite Panetti and Evans, {Donald L.} and Ruebush, {Mary J.} and Paulus, {Leslie K.} and Donald Burgess and Hale, {Arthur H.}",

note = "Funding Information: {\textquoteright} This work was supported by a research grant from the Forsyth Cancer Service and a grant to the Bowman Gray School of Medicine, Cancer Center Support (Core) Grant (2-P30-CA 12197-08) from the National Institutes of Health, Bethesda, Maryland, the American Cancer Society IM-224, and National Institutes of Health, CA 26084. Arthur H. Hale is a recipient of a Research Career Development Award (AI00383). {\textquoteright} To whom all correspondence should be addressed at: Department of Microbiology, Bowman Gray School of Medicine, Winston-Salem, N.C. 27 103. {\textquoteright} Abbreviations used: Con A, concanavalin A; CTL, cytotoxic thymus-derived lymphocyte; HBSS, Hanks{\textquoteright} balanced salt solution; PBS, phosphate-buffered saline (0.15 M NaCI, 0.01 M potassium phosphate, pH 7.4); RBC, red blood cell; SEM, scanning electron microscopy; TEM; transmission electron microscopy.",

year = "1981",

month = oct,

doi = "10.1016/0008-8749(81)90463-9",

language = "English (US)",

volume = "64",

pages = "119--130",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Surface analysis of early and late MOPC-315-EL tumor cells

AU - Harris, David

AU - Bailey, Jennifer

AU - Lewis, Jon C.

AU - Grover, William H.

AU - Panetti, Marguerite

AU - Evans, Donald L.

AU - Ruebush, Mary J.

AU - Paulus, Leslie K.

AU - Burgess, Donald

AU - Hale, Arthur H.

N1 - Funding Information: ’ This work was supported by a research grant from the Forsyth Cancer Service and a grant to the Bowman Gray School of Medicine, Cancer Center Support (Core) Grant (2-P30-CA 12197-08) from the National Institutes of Health, Bethesda, Maryland, the American Cancer Society IM-224, and National Institutes of Health, CA 26084. Arthur H. Hale is a recipient of a Research Career Development Award (AI00383). ’ To whom all correspondence should be addressed at: Department of Microbiology, Bowman Gray School of Medicine, Winston-Salem, N.C. 27 103. ’ Abbreviations used: Con A, concanavalin A; CTL, cytotoxic thymus-derived lymphocyte; HBSS, Hanks’ balanced salt solution; PBS, phosphate-buffered saline (0.15 M NaCI, 0.01 M potassium phosphate, pH 7.4); RBC, red blood cell; SEM, scanning electron microscopy; TEM; transmission electron microscopy.

PY - 1981/10

Y1 - 1981/10

N2 - The surface properties of a tumor line which undergoes a cyclic change in reactivity with cytotoxic T lymphocytes (CTLs) have been analyzed. The findings indicate that MOPC-315-EL when transplanted into the peritoneum of a BALB/c mouse for 4-5 days (early MOPC315-EL) becomes sensitive to lysis by anti-H-2d CTL. After 9 to 10 days of growth in the peritoneal cavity these tumor cells (late MOPC-315-EL) become unreactive to CTLs. The amount of H-2 antigens on the surface of the cells is the same on the early and late tumor cells. By scanning electron microscopy (SEM) we have shown that the early population is a mixture of two cell types which are characterized by surface villi or surface blebs; the late cells are essentially one population which are characterized by surface villi. The net charge on the surface of the cell was evaluated by two methods: (i) rate of cell migration across an electric potential and (ii) binding by cationic ferritin to the glycocalyx. Both techniques indicated that the early cells have a higher net negative charge. The implication of this work is that surface charge may be involved in T cell-target cell interactions.

AB - The surface properties of a tumor line which undergoes a cyclic change in reactivity with cytotoxic T lymphocytes (CTLs) have been analyzed. The findings indicate that MOPC-315-EL when transplanted into the peritoneum of a BALB/c mouse for 4-5 days (early MOPC315-EL) becomes sensitive to lysis by anti-H-2d CTL. After 9 to 10 days of growth in the peritoneal cavity these tumor cells (late MOPC-315-EL) become unreactive to CTLs. The amount of H-2 antigens on the surface of the cells is the same on the early and late tumor cells. By scanning electron microscopy (SEM) we have shown that the early population is a mixture of two cell types which are characterized by surface villi or surface blebs; the late cells are essentially one population which are characterized by surface villi. The net charge on the surface of the cell was evaluated by two methods: (i) rate of cell migration across an electric potential and (ii) binding by cationic ferritin to the glycocalyx. Both techniques indicated that the early cells have a higher net negative charge. The implication of this work is that surface charge may be involved in T cell-target cell interactions.

UR - http://www.scopus.com/inward/record.url?scp=0019464031&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019464031&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(81)90463-9

DO - 10.1016/0008-8749(81)90463-9

M3 - Article

C2 - 7296672

AN - SCOPUS:0019464031

SN - 0008-8749

VL - 64

SP - 119

EP - 130

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1

ER -

Surface analysis of early and late MOPC-315-EL tumor cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this