TY - JOUR
T1 - Suppression of cytochrome P450 reductase expression promotes astrocytosis in subventricular zone of adult mice
AU - Yao, Yunyi
AU - Liu, Senyan
AU - Wang, Yue
AU - Yuan, Weiping
AU - Ding, Xinxin
AU - Cheng, Tao
AU - Shen, Qin
AU - Gu, Jun
N1 - Funding Information:
We gratefully acknowledge the use of the Biochemistry, Advanced Light Microscopy, and Histopathology Core facilities of the Wadsworth Center. This work was supported in part by funds from the MOST grants 2011CB964801/2011ZX09102-1004/2012CB966604 and NNSF 81090410/81130074 (to T.C. or W.Y.), Neural Stem Cell Institute (to Q.S.) and NIH grants CA092596 (to X.D.), and AG026329 (to J.G.).
PY - 2013/8/26
Y1 - 2013/8/26
N2 - The aim of this study was to determine the role of NADPH-cytochrome P450 reductase (CPR) and CPR-dependent enzymes in neural stem cell (NSC) genesis in the brain. A mouse model with globally suppressed Cpr gene expression (Cpr-low mouse) was studied for this purpose. Cpr-low and wild-type (WT) mice were compared immunohistochemically for the expression of markers of cell proliferation (Ki67), immature neurons (doublecortin, DCX), oligodendrocytes (oligodendrocyte transcription factor 2, OLIG2), and astrocytes (glial fibrillary acidic protein, GFAP) in the SVZ, and for the in vitro capability of their SVZ cells to form neurospheres and differentiate into astrocytes. We found that the abundance of SVZ cells that are positive for Ki67 or GFAP expression, but not the abundance of SVZ cells that are positive for DCX and OLIG2 expression, was significantly increased in Cpr-low mice, at various ages, compared with WT mice. Furthermore, extents of astrocyte differentiation and growth, but not neurosphere formation, from SVZ cells of the Cpr-low mice were significantly increased, compared with WT mice. These results suggest that CPR and CPR-dependent enzymes play a role in suppressing astrocytosis in the SVZ of adult mice.
AB - The aim of this study was to determine the role of NADPH-cytochrome P450 reductase (CPR) and CPR-dependent enzymes in neural stem cell (NSC) genesis in the brain. A mouse model with globally suppressed Cpr gene expression (Cpr-low mouse) was studied for this purpose. Cpr-low and wild-type (WT) mice were compared immunohistochemically for the expression of markers of cell proliferation (Ki67), immature neurons (doublecortin, DCX), oligodendrocytes (oligodendrocyte transcription factor 2, OLIG2), and astrocytes (glial fibrillary acidic protein, GFAP) in the SVZ, and for the in vitro capability of their SVZ cells to form neurospheres and differentiate into astrocytes. We found that the abundance of SVZ cells that are positive for Ki67 or GFAP expression, but not the abundance of SVZ cells that are positive for DCX and OLIG2 expression, was significantly increased in Cpr-low mice, at various ages, compared with WT mice. Furthermore, extents of astrocyte differentiation and growth, but not neurosphere formation, from SVZ cells of the Cpr-low mice were significantly increased, compared with WT mice. These results suggest that CPR and CPR-dependent enzymes play a role in suppressing astrocytosis in the SVZ of adult mice.
KW - Astrocytosis
KW - Cytochrome P450 reductase
KW - Mice
KW - Neural stem cells
KW - Subventricular zone
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U2 - 10.1016/j.neulet.2013.05.043
DO - 10.1016/j.neulet.2013.05.043
M3 - Article
C2 - 23727388
AN - SCOPUS:84880136107
SN - 0304-3940
VL - 548
SP - 84
EP - 89
JO - Neuroscience Letters
JF - Neuroscience Letters
ER -