Superoxide dismutase activity in Alzheimer's disease: possible mechanism for paired helical filament formation

Frank P. Zemlan; Ole J. Thienhaus; H. Bruce Bosmann

doi:10.1016/0006-8993(89)91550-3

Superoxide dismutase activity in Alzheimer's disease: possible mechanism for paired helical filament formation

Frank P. Zemlan, Ole J. Thienhaus, H. Bruce Bosmann

Psychiatry

Research output: Contribution to journal › Article › peer-review

167 Scopus citations

Abstract

Activity of the free radical scavenging enzyme, superoxide dismutase (SOD-1), was determined in fibroblast cell lines derived from familial Alzheimer's patients, trisomy 21 patients and normal controls. In the present study, SOD-1 activity was significantly elevated by 30% in Alzheimer's cell lines when compared to normal euploid cell lines. As SOD-1 activity is known to be elevated about 50% in trisomy 21 patients, these cell lines were included as a control for tissue culture and assay conditions. In the present study, SOD-1 activity was significantly increased by 42±11% in trisomy 21 patients. The elevation in SOD-1 activity observed in the familial Alzheimer's patients supports the theory that paired helical filaments are synthesized in Alzheimer's disease by free radical hydroxylation of proline residues in paired helical filament precursor protein(s).

Original language	English (US)
Pages (from-to)	160-162
Number of pages	3
Journal	Brain Research
Volume	476
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(89)91550-3
State	Published - Jan 2 1989

Keywords

Alzheimer's disease
Free radical
Neurodegenerative disorder
Paired helical filament
Senile plaques
Superoxide dismutase

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(89)91550-3

Cite this

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title = "Superoxide dismutase activity in Alzheimer's disease: possible mechanism for paired helical filament formation",

abstract = "Activity of the free radical scavenging enzyme, superoxide dismutase (SOD-1), was determined in fibroblast cell lines derived from familial Alzheimer's patients, trisomy 21 patients and normal controls. In the present study, SOD-1 activity was significantly elevated by 30% in Alzheimer's cell lines when compared to normal euploid cell lines. As SOD-1 activity is known to be elevated about 50% in trisomy 21 patients, these cell lines were included as a control for tissue culture and assay conditions. In the present study, SOD-1 activity was significantly increased by 42±11% in trisomy 21 patients. The elevation in SOD-1 activity observed in the familial Alzheimer's patients supports the theory that paired helical filaments are synthesized in Alzheimer's disease by free radical hydroxylation of proline residues in paired helical filament precursor protein(s).",

keywords = "Alzheimer's disease, Free radical, Neurodegenerative disorder, Paired helical filament, Senile plaques, Superoxide dismutase",

author = "Zemlan, {Frank P.} and Thienhaus, {Ole J.} and Bosmann, {H. Bruce}",

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T1 - Superoxide dismutase activity in Alzheimer's disease

T2 - possible mechanism for paired helical filament formation

AU - Zemlan, Frank P.

AU - Thienhaus, Ole J.

AU - Bosmann, H. Bruce

PY - 1989/1/2

Y1 - 1989/1/2

N2 - Activity of the free radical scavenging enzyme, superoxide dismutase (SOD-1), was determined in fibroblast cell lines derived from familial Alzheimer's patients, trisomy 21 patients and normal controls. In the present study, SOD-1 activity was significantly elevated by 30% in Alzheimer's cell lines when compared to normal euploid cell lines. As SOD-1 activity is known to be elevated about 50% in trisomy 21 patients, these cell lines were included as a control for tissue culture and assay conditions. In the present study, SOD-1 activity was significantly increased by 42±11% in trisomy 21 patients. The elevation in SOD-1 activity observed in the familial Alzheimer's patients supports the theory that paired helical filaments are synthesized in Alzheimer's disease by free radical hydroxylation of proline residues in paired helical filament precursor protein(s).

AB - Activity of the free radical scavenging enzyme, superoxide dismutase (SOD-1), was determined in fibroblast cell lines derived from familial Alzheimer's patients, trisomy 21 patients and normal controls. In the present study, SOD-1 activity was significantly elevated by 30% in Alzheimer's cell lines when compared to normal euploid cell lines. As SOD-1 activity is known to be elevated about 50% in trisomy 21 patients, these cell lines were included as a control for tissue culture and assay conditions. In the present study, SOD-1 activity was significantly increased by 42±11% in trisomy 21 patients. The elevation in SOD-1 activity observed in the familial Alzheimer's patients supports the theory that paired helical filaments are synthesized in Alzheimer's disease by free radical hydroxylation of proline residues in paired helical filament precursor protein(s).

KW - Alzheimer's disease

KW - Free radical

KW - Neurodegenerative disorder

KW - Paired helical filament

KW - Senile plaques

KW - Superoxide dismutase

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Superoxide dismutase activity in Alzheimer's disease: possible mechanism for paired helical filament formation

Abstract

Keywords

ASJC Scopus subject areas

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