[3H]Pirenzepine identifies putative M1 muscarinic receptors in human stellate ganglia

Mark Watson; William R. Roeske; Peter C. Johnson; Henry I. Yamamura

doi:10.1016/0006-8993(84)90751-0

[³H]Pirenzepine identifies putative M₁ muscarinic receptors in human stellate ganglia

Mark Watson, William R. Roeske, Peter C. Johnson, Henry I. Yamamura

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The specific binding of [³H]pirenzepine ([³H]PZ) and [³H](-) quinuclidinyl benzilate ([³H](-)QNB) was investigated in homogenates of human stellate ganglia. [³H]PZ saturation isotherms yielded a K_d of 14 nM and B_max of 16.7 fmol/mg protein, while [³H](-)QNB binding curves yielded a K_d of 59 pM and B_max of 33.0 fmol/mg protein. This represents the greatest proportion of high affinity [³H]PZ labeling, relative to [³H](-)QNB, seen in any peripheral tissue examined thus far.

Original language	English (US)
Pages (from-to)	179-182
Number of pages	4
Journal	Brain Research
Volume	290
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(84)90751-0
State	Published - Jan 2 1984

Keywords

ganglia
human
muscarinic
pirenzepine
quinuclidinyl benzilate
receptors

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/0006-8993(84)90751-0

Cite this

@article{68966a6f305e4f0b86b916077e5b2adc,

title = "[3H]Pirenzepine identifies putative M1 muscarinic receptors in human stellate ganglia",

abstract = "The specific binding of [3H]pirenzepine ([3H]PZ) and [3H](-) quinuclidinyl benzilate ([3H](-)QNB) was investigated in homogenates of human stellate ganglia. [3H]PZ saturation isotherms yielded a Kd of 14 nM and Bmax of 16.7 fmol/mg protein, while [3H](-)QNB binding curves yielded a Kd of 59 pM and Bmax of 33.0 fmol/mg protein. This represents the greatest proportion of high affinity [3H]PZ labeling, relative to [3H](-)QNB, seen in any peripheral tissue examined thus far.",

keywords = "ganglia, human, muscarinic, pirenzepine, quinuclidinyl benzilate, receptors",

author = "Mark Watson and Roeske, {William R.} and Johnson, {Peter C.} and Yamamura, {Henry I.}",

note = "Funding Information: Portions of this study were supported by USPHS Grants MH-30626, MH-27257, Program Project Grant HL 20984, a RSDA Type II (MH-00095) from NIMH to H.I.Y., and a RSDA (HL-00776) from NHLBI to W.R.R. We thank Mrs. Pat Gonzalez for fine secretarial assistance, and Ms. Martha Acker-man for excellent technical help.",

year = "1984",

month = jan,

day = "2",

doi = "10.1016/0006-8993(84)90751-0",

language = "English (US)",

volume = "290",

pages = "179--182",

journal = "Brain Research",

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TY - JOUR

T1 - [3H]Pirenzepine identifies putative M1 muscarinic receptors in human stellate ganglia

AU - Watson, Mark

AU - Roeske, William R.

AU - Johnson, Peter C.

AU - Yamamura, Henry I.

N1 - Funding Information: Portions of this study were supported by USPHS Grants MH-30626, MH-27257, Program Project Grant HL 20984, a RSDA Type II (MH-00095) from NIMH to H.I.Y., and a RSDA (HL-00776) from NHLBI to W.R.R. We thank Mrs. Pat Gonzalez for fine secretarial assistance, and Ms. Martha Acker-man for excellent technical help.

PY - 1984/1/2

Y1 - 1984/1/2

N2 - The specific binding of [3H]pirenzepine ([3H]PZ) and [3H](-) quinuclidinyl benzilate ([3H](-)QNB) was investigated in homogenates of human stellate ganglia. [3H]PZ saturation isotherms yielded a Kd of 14 nM and Bmax of 16.7 fmol/mg protein, while [3H](-)QNB binding curves yielded a Kd of 59 pM and Bmax of 33.0 fmol/mg protein. This represents the greatest proportion of high affinity [3H]PZ labeling, relative to [3H](-)QNB, seen in any peripheral tissue examined thus far.

AB - The specific binding of [3H]pirenzepine ([3H]PZ) and [3H](-) quinuclidinyl benzilate ([3H](-)QNB) was investigated in homogenates of human stellate ganglia. [3H]PZ saturation isotherms yielded a Kd of 14 nM and Bmax of 16.7 fmol/mg protein, while [3H](-)QNB binding curves yielded a Kd of 59 pM and Bmax of 33.0 fmol/mg protein. This represents the greatest proportion of high affinity [3H]PZ labeling, relative to [3H](-)QNB, seen in any peripheral tissue examined thus far.

KW - ganglia

KW - human

KW - muscarinic

KW - pirenzepine

KW - quinuclidinyl benzilate

KW - receptors

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